Construction and characterization of a full-length infectious clone of an emerging senecavirus A strain

被引：1

作者：

Wang, Hao ^{[1
]}

Mo, Yongfang ^{[1
]}

Liu, Wenbo ^{[1
]}

Niu, Chenxia ^{[1
]}

He, Qijie ^{[1
]}

Ren, Tongwei ^{[1
]}

Ouyang, Kang ^{[1
,2
,3
,4
]}

Chen, Ying ^{[1
,2
,3
,4
]}

Huang, Weijian ^{[1
,2
,3
,4
]}

Wei, Zuzhang ^{[1
,2
,3
,4
]}

机构：

[1] Guangxi Univ, Coll Anim Sci & Technol, Lab Anim Infect Dis & Mol Immunol, Nanning 530005, Peoples R China

[2] Guangxi Zhuang Autonomous Reg Engn Res Ctr Vet Bio, Nanning 530004, Peoples R China

[3] Guangxi Key Lab Anim Reprod Breeding & Dis Control, Nanning 530005, Peoples R China

[4] Guangxi Coll & Univ Key Lab Prevent & Control Anim, Nanning 530005, Peoples R China

来源：

ARCHIVES OF VIROLOGY | 2024年 / 169卷 / 02期

关键词：

VESICULAR DISEASE;

D O I：

10.1007/s00705-023-05951-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Senecavirus A (SVA) is an emerging virus that causes vesicular disease in pigs. Construction of a full-length SVA cDNA clone is crucial for understanding its replication and pathogenesis. Here, we successfully constructed a CMV-promoter-driven infectious cDNA clone of the SVA isolate SVA/GX/CH/2018, which we named rSVA GX01. Sequence comparison between the pSVA GX01 and the parental isolate (SVA/GX/CH/2018) revealed three single-nucleotide differences. Four-week-old piglets were experimentally infected with either the parental virus or the cloned virus. The results showed that the cloned rSVA GX01 displayed weak pathogenicity in 4-week-old pigs compared to the parental virus SVA CH-GX-01-2018. The infectious clone of SVA will serve as a valuable tool for studying the viral replication cycle and for functional analysis of the viral genome.

引用

页数：8

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