Humanized Mouse Model of HIV Infection

被引：0
|
作者：
Leontyev, D. S. [1 ]
Glazkova, D. V. [1 ]
Bezborodova, O. A. [3 ]
Tsyganova, G. M. [1 ]
Urusov, F. A. [1 ,2 ]
Pankratov, A. A. [3 ]
Shipulin, G. A. [1 ]
Bogoslovskaya, E. V. [1 ]
机构：
[1] Fed Med Biol Agcy Russia, Ctr Strateg Planning & Management Biomed Hlth Risk, Moscow, Russia
[2] NF Izmerov Res Inst Occupat Hlth, Moscow, Russia
[3] Minist Hlth Russian Federat, Natl Med Res Radiol Ctr, PA Hertsen Moscow Oncol Res Inst, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2023年 / 175卷 / 1期
关键词：
humanized mice; HIV; in vivo; hematopoietic stem cells; animal models;
D O I：
10.1007/s10517-023-05812-3
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The development of new drugs for the treatment of HIV infection requires testing of their efficacy in a relevant animal model, such as humanized mice, which, unfortunately, are not yet available in Russia. In the present study, we have developed conditions for the humanization of immunodeficient NSG mice with human hematopoietic stem cells. Humanized animals generated during the study showed a high degree of chimerism and harbored repopulation of the entire range of human lymphocytes required for HIV replication in the blood and organs. Inoculation of these mice with HIV-1 virus led to stable viremia, which was confirmed by the presence of viral RNA in blood plasma throughout the entire period of observation and proviral DNA in the organs of animals 4 weeks after HIV infection.
引用
收藏
页码：63 / 66
页数：4
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