Humanized Mouse Model of HIV Infection

被引：0
|
作者：
Leontyev, D. S. [1 ]
Glazkova, D. V. [1 ]
Bezborodova, O. A. [3 ]
Tsyganova, G. M. [1 ]
Urusov, F. A. [1 ,2 ]
Pankratov, A. A. [3 ]
Shipulin, G. A. [1 ]
Bogoslovskaya, E. V. [1 ]
机构：
[1] Fed Med Biol Agcy Russia, Ctr Strateg Planning & Management Biomed Hlth Risk, Moscow, Russia
[2] NF Izmerov Res Inst Occupat Hlth, Moscow, Russia
[3] Minist Hlth Russian Federat, Natl Med Res Radiol Ctr, PA Hertsen Moscow Oncol Res Inst, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2023年 / 175卷 / 1期
关键词：
humanized mice; HIV; in vivo; hematopoietic stem cells; animal models;
D O I：
10.1007/s10517-023-05812-3
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The development of new drugs for the treatment of HIV infection requires testing of their efficacy in a relevant animal model, such as humanized mice, which, unfortunately, are not yet available in Russia. In the present study, we have developed conditions for the humanization of immunodeficient NSG mice with human hematopoietic stem cells. Humanized animals generated during the study showed a high degree of chimerism and harbored repopulation of the entire range of human lymphocytes required for HIV replication in the blood and organs. Inoculation of these mice with HIV-1 virus led to stable viremia, which was confirmed by the presence of viral RNA in blood plasma throughout the entire period of observation and proviral DNA in the organs of animals 4 weeks after HIV infection.
引用
收藏
页码：63 / 66
页数：4
相关论文
共 50 条
  • [1] Humanized Mouse Model of HIV Infection
    D. S. Leontyev
    D. V. Glazkova
    O. A. Bezborodova
    G. M. Tsyganova
    F. A. Urusov
    A. A. Pankratov
    G. A. Shipulin
    E. V. Bogoslovskaya
    Bulletin of Experimental Biology and Medicine, 2023, 175 : 63 - 66
  • [2] Humanized Mouse Models of HIV Infection
    Denton, Paul W.
    Garcia, J. Victor
    AIDS REVIEWS, 2011, 13 (03) : 135 - 148
  • [3] HIV-1 infection and pathogenesis in a novel humanized mouse model
    Zhang, Liguo
    Kovalev, Grigoriy I.
    Su, Lishan
    BLOOD, 2007, 109 (07) : 2978 - 2981
  • [4] Humanized mouse models of latent HIV infection
    Deruaz, Maud
    Tager, Andrew M.
    CURRENT OPINION IN VIROLOGY, 2017, 25 : 97 - 104
  • [5] A novel humanized mouse model for HIV and tuberculosis co-infection studies
    Bohorquez, Jose Alejandro
    Adduri, Sitaramaraju
    Ansari, Danish
    John, Sahana
    Florence, Jon
    Adejare, Omoyeni
    Singh, Gaurav
    Konduru, Nagarjun V.
    Jagannath, Chinnaswamy
    Yi, Guohua
    FRONTIERS IN IMMUNOLOGY, 2024, 15
  • [6] A humanized mouse model to study NK cell biology during HIV infection
    Kim, Jocelyn T.
    Zack, Jerome A.
    JOURNAL OF CLINICAL INVESTIGATION, 2022, 132 (24):
  • [7] Humanized Mouse Models for The In Vivo Analysis of HIV Infection
    Garcia-Martinez, J. Victor
    JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2009, 51 : 79 - 79
  • [8] Neutralizing anti-HIV antibodies develop in a humanized mouse model of HIV-1 infection
    Seung, Edward
    Dudek, Timothy
    Dugast, Anne-Sophie
    Mattoo, Hamid
    Vrbanac, Vladimir
    Tivey, Trevor
    Cariappa, Annaiah
    Pillai, Shiv
    Luster, Andrew
    Tager, Andrew
    JOURNAL OF IMMUNOLOGY, 2012, 188
  • [9] Neutralizing anti-HIV antibodies develop in a humanized mouse model of HIV-1 infection
    Seung, E.
    Dugast, A.
    Dudek, T.
    Mattoo, H.
    Vrbanac, V.
    Tivey, T.
    Murooka, T.
    Cariappa, A.
    Luster, A. D.
    Pillai, S.
    Tager, A. M.
    RETROVIROLOGY, 2012, 9
  • [10] Neutralizing anti-HIV antibodies develop in a humanized mouse model of HIV-1 infection
    E Seung
    A Dugast
    T Dudek
    H Mattoo
    V Vrbanac
    T Tivey
    T Murooka
    A Cariappa
    AD Luster
    S Pillai
    AM Tager
    Retrovirology, 9
12345