Pathogenic NKT cells attenuate urogenital chlamydial clearance and enhance infertility

被引:2
|
作者
Armitage, Charles W.
Carey, Alison J.
Bryan, Emily R.
Kollipara, Avinash
Trim, Logan K.
Beagley, Kenneth W.
机构
[1] Queensland Univ Technol, Ctr Immunol & Infect Control, Brisbane, Qld, Australia
[2] Queensland Univ Technol, Sch Biomed Sci, Brisbane, Qld, Australia
基金
英国医学研究理事会; 澳大利亚国家健康与医学研究理事会;
关键词
CD1d; chlamydia; infertility; lipid; NKT; KILLER T-CELLS; TRACHOMATIS INFECTION; PROTECTIVE ROLE; IMMUNITY; SUBSETS; IMMUNOSURVEILLANCE; RECOGNITION; SUFFICIENT; MODULATE;
D O I
10.1111/sji.13263
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Urogenital chlamydial infections continue to increase with over 127 million people affected annually, causing significant economic and public health pressures. While the role of traditional MHCI and II peptide presentation is well defined in chlamydial infections, the role of lipid antigens in immunity remains unclear. Natural killer (NK) T cells are important effector cells that recognize and respond to lipid antigens during infections. Chlamydial infection of antigen-presenting cells facilitates presentation of lipid on the MHCI-like protein, CD1d, which stimulates NKT cells to respond. During urogenital chlamydial infection, wild-type (WT) female mice had significantly greater chlamydial burden than CD1d(-/-) (NKT-deficient) mice, and had significantly greater incidence and severity of immunopathology in both primary and secondary infections. WT mice had similar vaginal lymphocytic infiltrate, but 59% more oviduct occlusion compared to CD1d(-/-) mice. Transcriptional array analysis of oviducts day 6 post-infection revealed WT mice had elevated levels of Ifn gamma (6-fold), Tnf alpha (38-fold), Il6 (2.5-fold), Il1 beta (3-fold) and Il17a (6-fold) mRNA compared to CD1d(-/-) mice. In infected females, oviduct tissues had an elevated infiltration of CD4(+)-invariant NKT (iNKT) cells, however, iNKT-deficient J alpha 18(-/-) mice had no significant differences in hydrosalpinx severity or incidence compared to WT controls. Lipid mass spectrometry of surface-cleaved CD1d in infected macrophages revealed an enhancement of presented lipids and cellular sequestration of sphingomyelin. Taken together, these data suggest an immunopathogenic role for non-invariant NKT cells in urogenital chlamydial infections, facilitated by lipid presentation via CD1d via infected antigen-presenting cells.
引用
收藏
页数:16
相关论文
共 50 条
  • [41] Mesenchymal stem cells with overexpression of midkine enhance cell survival and attenuate cardiac dysfunction in a rat model of myocardial infarction
    Shu-Li Zhao
    Yao-Jun Zhang
    Ming-Hui Li
    Xin-Lei Zhang
    Shao-Liang Chen
    Stem Cell Research & Therapy, 5
  • [42] Helicobacter pylori CagA mediated mitophagy to attenuate the NLRP3 inflammasome activation and enhance the survival of infected cells
    Chen, Dingyu
    Wu, Lixia
    Liu, Xi
    Wang, Qinrong
    Gui, Shuqin
    Bao, Liya
    Wang, Zhengrong
    He, Xiaofeng
    Zhao, Yan
    Zhou, Jianjiang
    Xie, Yuan
    SCIENTIFIC REPORTS, 2024, 14 (01):
  • [43] Hypoxia-expanded mesenchymal stromal cells enhance the angiogenesis and attenuate hindlimb ischemia via Gremlin1
    Huang, X.
    Zhao, L.
    Wei, C.
    CYTOTHERAPY, 2020, 22 (05) : S112 - S112
  • [44] Mesenchymal stem cells enhance microglia M2 polarization and attenuate neuroinflammation through TSG-6
    Liu, Yi
    Zeng, Rong
    Wang, Yezhong
    Huang, Wenhui
    Hu, Bin
    Zhu, Guohui
    Zhang, Run
    Li, Feng
    Han, Jianbang
    Li, Yongshi
    BRAIN RESEARCH, 2019, 1724
  • [45] Mesenchymal stem cells with overexpression of midkine enhance cell survival and attenuate cardiac dysfunction in a rat model of myocardial infarction
    Zhao, Shu-Li
    Zhang, Yao-Jun
    Li, Ming-Hui
    Zhang, Xin-Lei
    Chen, Shao-Liang
    STEM CELL RESEARCH & THERAPY, 2014, 5
  • [46] Neutrophils enhance clearance of necrotic epithelial cells in ozone-induced lung injury in rhesus monkeys
    Hyde, DM
    Miller, LA
    McDonald, RJ
    Stovall, MY
    Wong, V
    Pinkerton, KE
    Wegner, CD
    Rothlein, R
    Plopper, CG
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1999, 277 (06) : L1190 - L1198
  • [47] Polyvinylpyrrolidone capped silver nanoparticles enhance the autophagic clearance of Acinetobacter baumannii from human pulmonary cells
    Sharma, Saroj
    Tiwari, Vishvanath
    DISCOVER NANO, 2024, 19 (01)
  • [48] Evidence that human NKT cells enhance haemopoiesis through recognition of CD1d expressed in haemopoietic stem cells with long term clonogenic capacity
    Kotsianidis, L
    Patterson, S
    Politou, M
    Almeida, A
    Pantelidou, D
    Tsatalas, C
    Bourikas, G
    Roberts, I
    Karadimitris, A
    BLOOD, 2004, 104 (11) : 119B - 119B
  • [49] NKT cells enhance CD4+ and CD8+ T cell responses to soluble antigen in vivo through direct interaction with dendritic cells
    Hermans, IF
    Silk, JD
    Gileadi, U
    Salio, M
    Mathew, B
    Ritter, G
    Schmidt, R
    Harris, AL
    Old, L
    Cerundolo, V
    JOURNAL OF IMMUNOLOGY, 2003, 171 (10): : 5140 - 5147
  • [50] Pathogenic Intestinal Bacteria Enhance Prostate Cancer Development via Systemic Activation of Immune Cells in Mice
    Poutahidis, Theofilos
    Cappelle, Kelsey
    Levkovich, Tatiana
    Lee, Chung-Wei
    Doulberis, Michael
    Ge, Zhongming
    Fox, James G.
    Horwitz, Bruce H.
    Erdman, Susan E.
    PLOS ONE, 2013, 8 (08):