Thiol Quantification Using Colorimetric Thiol-Disulfide Exchange in Nonaqueous Solvents

被引：3
|
作者：
Schmidt, Ryan [1 ]
Logan, Matthew G. [1 ]
Patty, Sarah [1 ]
Ferracane, Jack L. [1 ]
Pfeifer, Carmem S. [1 ]
Kendall, Alexander J. [1 ]
机构：
[1] Oregon Hlth & Sci Univ, Sch Dent, Biomat & Biomech, Portland, OR 97201 USA
来源：
ACS OMEGA | 2023年 / 8卷 / 10期
基金：
美国国家科学基金会;
关键词：
ELLMANS REAGENT; REDUCTION; ASSAY; VERSATILE; TOOLS;
D O I：
10.1021/acsomega.2c07792
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A careful analysis of two (thiol-disulfide exchange) thiol quantification chromophores' behavior (Ellman's reagent and Aldrithiol-4) in nonaqueous solvents is presented. A wide range of kinetic profiles and response factors were measured to exhibit a large variance for nonaqueous systems. We report several robust benchtop and room-temperature methods using different organic solvents compared to aqueous conditions. Validation of analytical analyses in nonaqueous systems and quantification of the cysteine content of ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromo-phores under nonaqueous conditions for the quantification of thiols.
引用
收藏
页码：9356 / 9363
页数：8
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