The effect of high intensity interval training with genistein supplementation on mitochondrial function in the heart tissue of elderly rats

Introduction: For the most part, heart disease increases with age; on the other hand, although the role of exercise and antioxidants in the health of the elderly has been reported, the simultaneous effect of these two interventions is a new research topic. Thus, the aim of this study was to evaluate the effect of eight weeks of high intensity interval training (HIIT) and genistein (G) supplementation on oxidative stress, apoptosis and mitochondrial biogenesis in the heart tissue of elderly rats.Methods: In this experimental study, 40 elderly female Sprague-Dawley rats aged 20 +/- 2 months and weighing 250 +/- 30 g were randomly divided into five groups of eight animals, including: (1) control (C), (2) sham (Sh), (3) HIIT, (4) HIIT + G and (5) G. Also, to evaluate the effect of time passage on the variables, 8 healthy young rats were included in the healthy young control group. HIIT protocol was performed for eight weeks, three sessions with an intensity of 95-90 % VO2max at high intensity intervals and 45-45 % VO2max at low intensity intervals. Ge was received daily at a dose of 60 mg/kg peritoneally. Data analysis was performed using one-way ANOVA with Tukey's post hoc test (P <= 0.05).Results: In the HIIT and HIIT + G groups, levels of Bax, Bax/Bcl-2 ratio, MDA, PAB, GSSG were lower and levels of PGC-1 alpha, TFAM, GSH, GSH/GSSG ratio and NDUFS7 were higher than the control and sham groups (P <= 0.05). In the HIIT + G group, levels of Bcl-2 were significantly higher than the control group (P <= 0.05). In the HIIT + G group, levels of Bax, GSSG, Bax/Bcl-2 ratio, and PAB were lower, and levels of GSH/GSSG ratio, Bcl-2, PGC-1 alpha, TFAM and NDUFS7 were higher than the G consumption group (P <= 0.05). In the HIIT + G group, levels of PGC-1 alpha and TFAM were significantly higher and levels of MDA and PAB were lower than the HIIT group (P <= 0.05).Conclusion: Both HIIT and G consumption seem to have beneficial effects on reducing oxidative stress; in addi-tion, the interaction of these two variables on the improvement of apoptosis and mitochondrial biogenesis is more favorable than the effect of either one alone. However, more studies are needed on different pathways of apoptosis following G administration.