Subsurface constructed wetlands with modified biochar added for advanced treatment of tailwater: Performance and microbial communities

The limitations of conventional substrates in treating wastewater treatment plant tailwater are evident in sub-surface flow constructed wetlands, and the emergence of biochar presents a solution to this problem. The objective of this study was to assess and prioritize the efficacy of various modified reed biochar in removing pollutants when used as fillers in wetland systems. To achieve this, we established multiple simulation systems of vertical groundwater flow wetlands, each filled with different modified reed biochar. The reed biochar was prepared and modified using Pingluo reed poles from Ningxia. We monitored the quality of the effluent water and the diversity of the microbial community in order to evaluate the pollutant removal performance of the modified biochar under different hydraulic retention times in a laboratory setting. The findings indicated that a hydraulic retention time of 24-48 h was found to be optimal for each wetland system. Furthermore, the composite modified biochar system with KMnO4 and ZnCl2 exhibited higher levels of dissolved oxygen and lower conductivity, resulting in superior pollutant removal performance. Specifically, the system achieved removal rates of 89.94 % for COD, 85.88 % for TP, 91.05 % for TN, and 92.76 % for NH3-N. Additionally, the 16S rRNA high-throughput sequencing analysis revealed that the system displayed high Chao1, Shannon, and Simpson indices of 6548.75, 10.1965, and 0.9944, respectively. The predominant bacterial phyla observed in the wetland system were Proteobacteria, Bacteroidetes, Chloroflexi, Patescibacteria, Firmicutes, and Actinobacteria. Addi-tionally, the denitrifying bacterial class, Rhodobacteriaceae, was found to have the highest content ratio in this system. This finding serves as confirmation that the KMnO4 and ZnCl2 composite modified biochar can significantly enhance water purification performance. Consequently, this study offers valuable insights for wastewater treatment plants seeking to implement vertical submersible artificial wetland tailwater improvement projects.