High-Resolution Microscopic Ghost Imaging for Bioimaging

A label-free, low-photodamage, low-cost, and in vivo high-resolution microscopic imaging technique has been challenging to develop. Ghost imaging (GI) is a computational imaging protocol with the spa-tially nonlocal property, which enables low-light imaging. Considering the advantages of GI, we propose a method, called high-resolution microscopic ghost imaging (HRMGI), to achieve high-resolution imag-ing by simply demagnifying the signal beam. The analytical formulas for resolution and depth of field (DOF) of HRMGI are provided. Experimental results show that the HRMGI system can have 10.5 times higher resolution than the conventional GI, which can exceed the pixel size limit of multipixel detectors, and 58 times greater DOF than the traditional diffraction-limited optical (TDLO) system. To present the performance of HRMGI, blood vessels within an ovarian section are also imaged. The proposed method is expected to facilitate the development of bioimaging techniques and promote the practical application of GI.