Preparation of Polyclonal Antibody against ZmBT1 Protein and Its Application in Hormone-Regulated Starch Synthesis

被引：3
|
作者：
Liu, Lun [1 ]
Qing, Yun [2 ]
Shoaib, Noman [3 ,4 ,5 ]
Di, Runze [2 ]
Liu, Hanmei [6 ]
Li, Yangping [1 ]
Hu, Yufeng [1 ]
Huang, Yubi [1 ,2 ]
Yu, Guowu [1 ,2 ]
机构：
[1] Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu 611130, Peoples R China
[2] Sichuan Agr Univ, Coll Agron, Natl Demonstrat Ctr Expt Crop Sci Educ, Chengdu 611130, Peoples R China
[3] Chinese Acad Sci, CAS Key Lab Mt Ecol Restorat & Bioresource Utiliza, Chengdu 610041, Peoples R China
[4] Chinese Acad Sci, Chengdu Inst Biol, Ecol Restorat Biodivers Conservat Key Lab Sichuan, Chengdu 610041, Peoples R China
[5] Univ Chinese Acad Sci, Beijing 101408, Peoples R China
[6] Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Peoples R China
来源：
AGRONOMY-BASEL | 2023年 / 13卷 / 07期
基金：
中国国家自然科学基金;
关键词：
ZmBT1; polyclonal antibody preparation; starch synthesis; expression analysis; ADP-GLUCOSE PYROPHOSPHORYLASE; PROKARYOTIC EXPRESSION; TRANSCRIPTION FACTOR; KEY ENZYMES; GENE; MEMBRANE; ELEMENTS; BINDING; BIOSYNTHESIS; LOCALIZATION;
D O I：
10.3390/agronomy13071805
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
In order to investigate the crucial role of ZmBT1 in starch accumulation during maize grain development and analyze the expression and distribution of ZmBT1 in various maize tissues, we prepared a polyclonal antibody. Specifically, we successfully expressed the recombinant plasmid pGEX-6p-ZmBT1-C (382-437aa) and purified Gst-ZmBT1-C as the antigen for antibody preparation. Our results confirmed that the ZmBT1 protein in maize tissues can be specifically recognized by the ZmBT1 antibody. Through Western blotting, we observed that the expression protein of ZmBT1 varied by tissues, with the highest content in the grain and endosperm. Furthermore, we employed a combination of Western blotting and quantitative real-time PCR to show that the expression level of ZmBT1 can be influenced by plant hormones. This finding suggests that ZmBT1 plays a critical role in the accumulation of starch and opens up new avenues for functional studies of this protein.
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页数：15
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