Effects of Oxidative Stress on the Autophagy and Apoptosis of Granulosa Cells in Broody Geese

被引:12
|
作者
Hou, Li'E [1 ]
Gu, Tiantian [1 ]
Weng, Kaiqi [1 ]
Zhang, Yu [1 ]
Zhang, Yang [1 ]
Chen, Guohong [1 ]
Xu, Qi [1 ]
机构
[1] Yangzhou Univ, Jiangsu Key Lab Anim Genet Breeding & Mol Design, Yangzhou 225009, Peoples R China
关键词
goose; broodiness; oxidative stress; apoptosis; autophagy; VITAMIN-E; CASPASE ACTIVITY; REACTIVE OXYGEN; BROODINESS; FOLLICLES; BEHAVIOR;
D O I
10.3390/ijms24032154
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Broodiness is an unfavorable trait associated with the cessation of egg laying. Studies have found that excessive granulosa cell apoptosis and autophagy occur during goose broodiness. Other studies have also confirmed that oxidative stress is an important cause of apoptosis and autophagy. However, whether oxidative stress occurs during goose broodiness and whether this oxidative stress causes apoptosis and autophagy have not been fully elucidated. In this study, we investigated the effects of oxidative stress on the autophagy and apoptosis of granulosa cells in broody geese. The results showed higher mRNA expression of genes related to antioxidative stress responses (GPX, SOD-1, SOD-2, COX-2, CAT and hsp70) in pre-broody and broody geese than in laying birds. In addition, increased levels of granulosa cell apoptosis and autophagy were observed in pre-broody geese than in laying geese. Additionally, granulosa cells treated with H2O2 exhibited increased apoptosis and autophagy in vitro, and these effects were responsible for goose granulosa cell death. Moreover, vitamin E treatment effectively protected granulosa cells from H2O2-induced oxidative stress by inhibiting ROS production. Correspondingly, granulosa cell apoptosis and autophagy were greatly alleviated by vitamin E treatment. Together, our results demonstrated serious oxidative stress and granulosa cell apoptosis and autophagy in broody geese, and oxidative stress promoted apoptosis and autophagy. Vitamin E alleviated the autophagy and apoptosis of granulosa cells by inhibiting oxidative stress.
引用
收藏
页数:19
相关论文
共 50 条
  • [21] Silver nanoparticles induce oxidative stress, apoptosis and impaired steroidogenesis in ovarian granulosa cells of cattle
    Tabandeh, Mohammad Reza
    Samie, Kosar Abbasi
    Mobarakeh, Erfan Sadeghi
    Khadem, Mohammad Darvish
    Jozaie, Sadegh
    ANIMAL REPRODUCTION SCIENCE, 2022, 236
  • [22] Oxidative stress modulates the expression of apoptosis-associated microRNAs in bovine granulosa cells in vitro
    Md Mahmodul Hasan Sohel
    Bilal Akyuz
    Yusuf Konca
    Korhan Arslan
    Serpil Sariozkan
    Mehmet Ulas Cinar
    Cell and Tissue Research, 2019, 376 : 295 - 308
  • [23] Effects of Melatonin on the Proliferation and Apoptosis of Sheep Granulosa Cells under Thermal Stress
    Fu, Yao
    He, Chang-Jiu
    Ji, Peng-Yun
    Zhuo, Zhi-Yong
    Tian, Xiu-Zhi
    Wang, Feng
    Tan, Dun-Xian
    Liu, Guo-Shi
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (11): : 21090 - 21104
  • [24] Cisplatin and resveratrol induce apoptosis and autophagy following oxidative stress in malignant mesothelioma cells
    Lee, Yoon-Jin
    Lee, Gina J.
    Yi, Sun Shin
    Heo, Su-Hak
    Park, Cho-Rung
    Nam, Hae-Seon
    Cho, Moon-Kyun
    Lee, Sang-Han
    FOOD AND CHEMICAL TOXICOLOGY, 2016, 97 : 96 - 107
  • [25] The Responses of Autophagy and Apoptosis to Oxidative Stress in Nucleus Pulposus Cells: Implications for Disc Degeneration
    Chen, Jiang-Wei
    Ni, Bin-Bin
    Li, Bo
    Yang, Yue-Hua
    Jiang, Sheng-Dan
    Jiang, Lei-Sheng
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2014, 34 (04) : 1175 - 1189
  • [26] Protective effects of Oviductus Ranae-containing serum on oxidative stress-induced apoptosis in rat ovarian granulosa cells
    Ling, Xiao-Mei
    Zhang, Xu-Hui
    Tan, Yan
    Yang, Jing-Jing
    Ji, Bo
    Wu, Xin-Rong
    Yi, Yan-kui
    Liang, Lei
    JOURNAL OF ETHNOPHARMACOLOGY, 2017, 208 : 138 - 148
  • [27] Effects of Ethanol on ARPE-19 cells: Autophagy and Oxidative Stress
    Bonet, Luis
    Flores-Bellver, Miguel
    Saez-Atienzar, Sara
    Martinez, Natalia
    Sancho-Pelluz, Javier
    Barcia, Jorge M.
    Jordan, Joaquin
    Galindo, Maria F.
    Romero, Francisco J.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2014, 55 (13)
  • [28] Effects of Gentiopicroside on Oxidative Stress and Apoptosis in Gastric Cancer Cells
    Wu, Xinyi
    Li, Suying
    An, Shuo
    JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, 2021, 11 (03) : 553 - 559
  • [29] Effects of butein on human osteosarcoma cell proliferation, apoptosis, and autophagy through oxidative stress
    Zhang, Pei
    Zhang, Jiale
    Quan, Huahong
    Chen, Pengtao
    Wang, Jingcheng
    Liang, Yuan
    HUMAN & EXPERIMENTAL TOXICOLOGY, 2022, 41
  • [30] Effects of curcumin on cyclosporine A-induced oxidative stress, autophagy, and apoptosis in rat heart
    Naderi, Roya
    Seyhani, Alireza
    Shirpoor, Alireza
    Jafari, Adele
    Eyvani, Kimia
    MOLECULAR BIOLOGY REPORTS, 2025, 52 (01)