Characterization of an Omnitrap-Orbitrap Platform Equipped with Infrared Multiphoton Dissociation, Ultraviolet Photodissociation, and Electron Capture Dissociation for the Analysis of Peptides and Proteins

被引：6
|
作者：
Smyrnakis, Athanasios [1 ]
Levin, Nikita [2 ,3 ]
Kosmopoulou, Mariangela [1 ]
Jha, Ajay [2 ,3 ]
Fort, Kyle [4 ]
Makarov, Alexander [4 ]
Papanastasiou, Dimitris [1 ]
Mohammed, Shabaz [2 ,5 ,6 ]
机构：
[1] NCSR Demokritos, Fasmatech Sci & Technol, Aghia Paraskevi 15341, Greece
[2] Rosalind Franklin Inst, Harwell Campus, Didcot OX11 0QX, England
[3] Univ Oxford, Dept Pharmacol, Oxford OX1 3QT, England
[4] Thermo Fisher Sci, D-28199 Bremen, Germany
[5] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
[6] Univ Oxford, Dept Chem, Oxford OX1 3TA, England
来源：
ANALYTICAL CHEMISTRY | 2023年 / 95卷 / 32期
基金：
英国工程与自然科学研究理事会;
关键词：
MOBILITY-MASS-SPECTROMETRY; TOP-DOWN CHARACTERIZATION; QUADRUPOLE ION-TRAP; STRUCTURAL-CHARACTERIZATION; ACTIVATION METHODS; 157; NM; FRAGMENTATION; IMPLEMENTATION; CHARGE; PHOTOFRAGMENTATION;
D O I：
10.1021/acs.analchem.3c01899
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We describe an instrument configuration based on theOrbitrap Exploris480 mass spectrometer that has been coupled to an Omnitrap platform.The Omnitrap possesses three distinct ion-activation regions thatcan be used to perform resonant-based collision-induced dissociation,several forms of electron-associated fragmentation, and ultravioletphotodissociation. Each section can also be combined with infraredmultiphoton dissociation. In this work, we demonstrate all these modesof operation in a range of peptides and proteins. The results showthat this instrument configuration produces similar data to previousimplementations of each activation technique and at similar efficiencylevels. We demonstrate that this unique instrument configuration isextremely versatile for the investigation of polypeptides.
引用
收藏
页码：12039 / 12046
页数：8
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