Mead acid production by disruption ofd12-desaturase gene in Mortierella alpina 1S-4

被引：1
|
作者：
Kikukawa, Hiroshi [1 ,2 ]
Ando, Akinori [1 ,3 ]
Hannya, Asuka [1 ]
Asras, Mohd Fazli Farida [1 ]
Okuda, Tomoyo [1 ]
Sakamoto, Takaiku [4 ]
Hara, Kiyotaka Y. [2 ]
Sakuradani, Eiji [4 ]
Ogawa, Jun [1 ,3 ]
机构：
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
[2] Univ Shizuoka, Grad Sch Nutr & Environm Sci, 52-1 Yada,Suruga Ku, Shizuoka 4228526, Japan
[3] Kyoto Univ, Res Inst Math Sci, Sakyo Ku, Kyoto 6068502, Japan
[4] Tokushima Univ, Grad Sch Technol Ind & Social Sci, 2-1 Minamijosanjima cho, Tokushima 7708513, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2023年 / 136卷 / 05期
基金：
日本科学技术振兴机构;
关键词：
Mortierella alpina 1S-4; Gene disruption; A12-desaturase; Polyunsaturated fatty acid; Mead acid; HUMAN LIG4 HOMOLOG; EICOSAPENTAENOIC ACID; OLEAGINOUS FUNGUS; 5,8,11-EICOSATRIENOIC ACID; LINOLENIC ACID; FATTY-ACIDS; OIL; TRANSFORMATION; ESTABLISHMENT; EFFICIENCY;
D O I：
10.1016/j.jbiosc.2023.08.001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
August Mead acid (MA; 20:3u9) is one of the u9 series of polyunsaturated fatty acids (PUFAs). MA is used to inhibit the inflammation of joints and is applied to the medicinal or health food field. We aimed to construct MA-producing strains with disruption of the D12-desaturase gene (D12ds) via an efficient gene-targeting system using the lig4-disrupted strain of Mortierella alpina 1S-4 as the host. The transformants showed a unique fatty acid composition that only comprised u9-PUFAs and saturated fatty acids, while u6-and u3-PUFAs were not detected, and the total composition of u9-PUFAs, including oleic acid (18:1u9), 18:2u9, 20:1u9, 20:2u9, and MA, was up to 68.4% of the total fatty acids. The MA production in the D12ds-disruptant reached 0.10 g/L (8.5%), which exceeded 0.050 g/L (4.6%) in the conventional D12ds-defective mutant JT-180.
引用
收藏
页码：353 / 357
页数：5
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