Generation of a PDGFRB-mCherry knock-in reporter human induced pluripotent stem cell line (KITi001-A-1), using CRISPR/Cas9 nuclease

被引：1

作者：

Jo, Seongyea ^{[1
,2
]}

Kim, Ji-Woo ^{[1
]}

Noh, Haneul ^{[1
]}

Kim, Hyemin ^{[1
]}

Kim, Jong-Hoon ^{[2
]}

Park, Han-Jin ^{[1
]}

机构：

[1] Korea Inst Toxicol, Dept Predict Toxicol, Daejeon, South Korea

[2] Korea Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Lab Stem Cells & Tissue Regenerat, Seoul, South Korea

来源：

STEM CELL RESEARCH | 2023年 / 69卷

关键词：

D O I：

10.1016/j.scr.2023.103081

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-beta), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-beta plays an essential role in early liver diseases, including liver fibrosis. Here, we generated a human induced pluripotent stem cell (iPSC) line, KITi001-A-1, using CRISPR/Cas9. This reporter iPSC line and its derivatives are useful for tracing PDGFR-beta-expressing cells and for screening for liver fibrosis-inducing compounds.

引用

页数：6

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