Generation of a PDGFRB-mCherry knock-in reporter human induced pluripotent stem cell line (KITi001-A-1), using CRISPR/Cas9 nuclease

被引：1
|
作者：
Jo, Seongyea [1 ,2 ]
Kim, Ji-Woo [1 ]
Noh, Haneul [1 ]
Kim, Hyemin [1 ]
Kim, Jong-Hoon [2 ]
Park, Han-Jin [1 ]
机构：
[1] Korea Inst Toxicol, Dept Predict Toxicol, Daejeon, South Korea
[2] Korea Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Lab Stem Cells & Tissue Regenerat, Seoul, South Korea
来源：
STEM CELL RESEARCH | 2023年 / 69卷
关键词：
D O I：
10.1016/j.scr.2023.103081
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
PDGFRB encodes platelet-derived growth factor receptor beta (PDGFR-beta), a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. It is required for the normal development of the vascular and nervous systems and rearrangement of the actin cytoskeleton. PDGFR-beta plays an essential role in early liver diseases, including liver fibrosis. Here, we generated a human induced pluripotent stem cell (iPSC) line, KITi001-A-1, using CRISPR/Cas9. This reporter iPSC line and its derivatives are useful for tracing PDGFR-beta-expressing cells and for screening for liver fibrosis-inducing compounds.
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页数：6
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