Isolation and Characterization of Mycoplasma ovipneumoniae Infecting Goats with Pneumonia in Anhui Province, China

被引:0
|
作者
Chen, Jiahong [1 ,2 ,3 ]
Wang, Shijia [2 ]
Dong, Dong [3 ]
Zhang, Zijun [2 ,3 ]
Huang, Yafeng [2 ,3 ]
Zhang, Yong [1 ]
机构
[1] Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China
[2] Anhui Agr Univ, Coll Anim Sci & Technol, Hefei 230036, Peoples R China
[3] Ctr Agr Technol Cooperat & Promot Dingyuan Cty, Dingyuan 233200, Peoples R China
来源
LIFE-BASEL | 2024年 / 14卷 / 02期
关键词
goats; pleuropneumonia; Mycoplasma; amplicon sequencing; BIGHORN SHEEP; MOLECULAR-DETECTION; IDENTIFICATION; ARGININI; STRAINS; DISEASE; LUNGS;
D O I
10.3390/life14020218
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mycoplasma ovipneumoniae (M. ovipneumoniae) causes a fatal infection in goats, leading to significant economic losses in the small-ruminant industry worldwide. The present study aimed to characterize the strains of M. ovipneumoniae infecting goats with pneumonia in Anhui Province, China. From November 2021 to January 2023, among 20 flocks, a total of 1320 samples (600 samples of unvaccinated blood, 400 nasal swabs, 200 samples of pleural fluid, and 120 samples of lung tissue) were obtained from goats with typical signs of pneumonia, such as a low growth rate, appetite suppression, increased temperature, discharge from the nose, and a cough. Necropsied goats showed increased pleural fluid, fibrinous pleuropneumonia, and attached localized pleural adhesions. M. ovipneumoniae isolated from the samples were subjected to an indirect hemagglutination test (IHA), PCR amplicon sequencing, phylogenetic analysis, and biochemical identification tests. The overall positivity rate of M. ovipneumoniae was 27.50%. Mycoplasmas were obtained from 80 (20.0%) nasal swabs, 21 (10.5%) pleural fluid samples, and 15 (12.5%) lung samples. PCR amplicon (288 bp) sequencing identified eight strains of M. ovipneumoniae. In a phylogenetic tree, the isolated strains were homologous to the standard strain M. ovipneumoniae Y-98 and most similar to M. ovipneumoniae FJ-SM. Local strains of M. ovipneumoniae were isolated from goats in Anhui province. The identified genomic features and population structure will promote further study of M. ovipneumoniae pathogenesis and could form the basis for vaccine and therapy development.
引用
收藏
页数:14
相关论文
共 50 条
  • [31] ISOLATION, PROPAGATION, AND CHARACTERIZATION STUDIES OF AN OVINE MYCOPLASMA RESPONSIBLE FOR PROLIFERATIVE INTERSTITIAL PNEUMONIA
    CARMICHAEL, LE
    STGEORGE, TD
    SULLIVAN, ND
    HORSFALL, N
    CORNELL VETERINARIAN, 1972, 62 (04): : 654 - +
  • [32] Isolation and preliminary characterization of a large Babesia sp. from sheep and goats in the eastern part of Gansu Province, China
    Qi Bai
    Guangyuang Liu
    Dekao Liu
    Jiaxing Ren
    Xing Li
    Parasitology Research, 2002, 88 : S16 - S21
  • [33] Identification and Characterization of Colletotrichum Species Associated With Camellia sinensis Anthracnose in Anhui Province, China
    Zhang, Lei
    Li, Xianghan
    Zhou, Yuanyuan
    Tan, Genjia
    Zhang, Lixin
    PLANT DISEASE, 2021, 105 (09) : 2649 - 2657
  • [34] Seroprevalence and genetic characterization of Toxoplasma gondii in cancer patients in Anhui Province, Eastern China
    Lin Wang
    Liu-yuan He
    De-di Meng
    Zhao-wu Chen
    He Wen
    Gong-si Fang
    Qing-li Luo
    Kai-quan Huang
    Ji-long Shen
    Parasites & Vectors, 8
  • [35] Genetic characterization and phylogenetic analysis of feline astrovirus from Anhui province in eastern China
    Yong Wang
    Xu Guo
    Yongqiu Cui
    Yu Zhou
    Kankan Yang
    Ziteng Fu
    Jianfei Sun
    Guangqing Liu
    Bangzhao Cheng
    Shudong Jiang
    Yongdong Li
    3 Biotech, 2020, 10
  • [36] Molecular characterization of Trichuris ovis from goats in hunan province of China
    Tang, Jia-Min
    Song, Lu-Lin
    Nie, Lin-Feng
    Li, Fen
    THAI JOURNAL OF VETERINARY MEDICINE, 2018, 48 (03): : 471 - 477
  • [37] Genetic characterization and phylogenetic analysis of feline astrovirus from Anhui province in eastern China
    Wang, Yong
    Guo, Xu
    Cui, Yongqiu
    Zhou, Yu
    Yang, Kankan
    Fu, Ziteng
    Sun, Jianfei
    Liu, Guangqing
    Cheng, Bangzhao
    Jiang, Shudong
    Li, Yongdong
    3 BIOTECH, 2020, 10 (08)
  • [38] Seroprevalence and genetic characterization of Toxoplasma gondii in cancer patients in Anhui Province, Eastern China
    Wang, Lin
    He, Liu-yuan
    Meng, De-di
    Chen, Zhao-wu
    Wen, He
    Fang, Gong-si
    Luo, Qing-li
    Huang, Kai-quan
    Shen, Ji-long
    PARASITES & VECTORS, 2015, 8
  • [39] ISOLATION AND CHARACTERIZATION OF MYCOPLASMA-CONJUNCTIVAE SP-N FROM SHEEP AND GOATS WITH KERATOCONJUNCTIVITIS
    BARILE, MF
    DELGIUDI.RA
    TULLY, JG
    INFECTION AND IMMUNITY, 1972, 5 (01) : 70 - &
  • [40] Primary surveys on molecular epidemiology of bovine viral diarrhea virus 1 infecting goats in Jiangsu province, China
    Mao, Li
    Li, Wenliang
    Yang, Leilei
    Wang, Jianhui
    Cheng, Suping
    Wei, Yong
    Wang, Qiusheng
    Zhang, Wenwen
    Hao, Fei
    Ding, Yonglong
    Sun, Yinhua
    Jiang, Jieyuan
    BMC VETERINARY RESEARCH, 2016, 12