Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets

被引：5
|
作者：
Khatir, Ines [1 ,2 ]
Brunet, Marie A. [3 ,4 ]
Meller, Anna [5 ]
Amiot, Florent [1 ,2 ]
Patel, Tushar [1 ,2 ]
Lapointe, Xavier [3 ,4 ]
Avila Lopez, Jessica [1 ,2 ]
Guilloy, Noe [2 ]
Castonguay, Anne [2 ]
Husain, Mohammed Amir [1 ,6 ]
St Germain, Joannie [1 ,2 ]
Boisvert, Francois-Michel [4 ,5 ]
Plourde, Melanie [1 ,6 ]
Roucou, Xavier [2 ,4 ,7 ]
Laurent, Benoit [1 ,2 ]
机构：
[1] Ctr Hosp Univ Sherbrooke, Res Ctr Aging, Ctr Integre Univ Sante & Serv Sociaux Estrie, Sherbrooke, PQ J1H 4C4, Canada
[2] Univ Sherbrooke, Fac Med & Hlth Sci, Dept Biochem & Funct Genom, Sherbrooke, PQ J1H 5N4, Canada
[3] Univ Sherbrooke, Dept Pediat, Med Genet Serv, Sherbrooke, PQ J1H 5N4, Canada
[4] CRCHUS, Sherbrooke, PQ J1H 5N4, Canada
[5] Univ Sherbrooke, Fac Med & Hlth Sci, Dept Immunol & Cell Biol, Quebec City, PQ J1H 5N4, Canada
[6] Univ Sherbrooke, Fac Med & Hlth Sci, Dept Med, Sherbrooke, PQ J1H 5N4, Canada
[7] PROTEO, Quebec Network Res Prot Funct Struct & Engn, Quebec City, PQ G1V 0A6, Canada
来源：
CELLS | 2023年 / 12卷 / 04期
基金：
加拿大健康研究院;
关键词：
aging; brain; cortex; mRNA; transcriptome; protein; proteome; CORTICAL THICKNESS; PROTEASOME; MATURATION; PLASTICITY; INITIATION; DEMENTIA; SUBUNIT; CORTEX; RATES;
D O I：
10.3390/cells12040615
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
During aging, changes in gene expression are associated with a decline in physical and cognitive abilities. Here, we investigate the connection between changes in mRNA and protein expression in the brain by comparing the transcriptome and proteome of the mouse cortex during aging. Our transcriptomic analysis revealed that aging mainly triggers gene activation in the cortex. We showed that an increase in mRNA expression correlates with protein expression, specifically in the anterior cingulate cortex, where we also observed an increase in cortical thickness during aging. Genes exhibiting an aging-dependent increase of mRNA and protein levels are involved in sensory perception and immune functions. Our proteomic analysis also identified changes in protein abundance in the aging cortex and highlighted a subset of proteins that were differentially enriched but exhibited stable mRNA levels during aging, implying the contribution of aging-related post- transcriptional and post-translational mechanisms. These specific genes were associated with general biological processes such as translation, ribosome assembly and protein degradation, and also important brain functions related to neuroplasticity. By decoupling mRNA and protein expression, we have thus characterized distinct subsets of genes that differentially adjust to cellular aging in the cerebral cortex.
引用
收藏
页数：21
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