Heterotrophic nitrification-aerobic denitrification performance of a novel strain, Pseudomonas sp. B-1, isolated from membrane aerated biofilm reactor

A heterotrophic nitrification-aerobic denitrification (HN-AD) strain isolated from membrane aerated biofilm reactor (MABR) was identified as Pseudomonas sp. B-1, which could effectively utilize multiple nitrogen sources and preferentially consume NH4-N. The maximum degradation efficiencies of NO3-N, NO2-N and NH4-N were 98.04%, 94.84% and 95.74%, respectively. The optimal incubation time, shaking speed, carbon source, pH, temperature and C/N ratio were 60 h, 180 rpm, sodium succinate, 8, 30 degrees C and 25, respectively. The strain preferred salinity of 1.5% and resisted heavy metals in the order of Mn2+ > Co2+ > Zn2+ > Cu2+. It can be preliminarily speculated from the results of enzyme assay that the strain removed nitrogen via full nitrification-denitrification pathway. The addition of strain into the conventional MABR significantly intensified the HN-AD performance of the reactor. The relative abundance of the functional bacteria including Flavobacterium, Pseu-domonas, Paracoccus, Azoarcus and Thauera was obviously increased after the bioaugmentation. Besides, the expression of the HN-AD related genes in the biofilm was also strengthened. Thus, strain B-1 had great appli-cation potential in nitrogen removal process.