Novel Flow Cytometry-Based Method for Detection of Anti-HLA Complement Activating Donor-Specific Antibodies in Renal Transplant Recipients and Its Comparison With Other Conventional Detection Methods

被引：1
|
作者：
Rani, Lekha [1 ]
Singh, Heera [1 ]
Saikia, Biman [1 ]
Aggarwal, Ritu [1 ]
Kumar, Yashwant [1 ]
Kumar, Mahendra [1 ]
Chhabra, Seema [1 ]
Kumar, Manoj [2 ]
Kumar, Bhuvnesh [2 ]
Kumar, Vinkesh [2 ]
Das, Prabir [1 ]
Sharma, Ashish [2 ]
Ramchandran, Raja [3 ]
Kohli, Harbir Singh [3 ]
Minz, Ranjana Walker [1 ,4 ]
机构：
[1] Postgrad Inst Med Educ & Res, Dept Immunopathol, Chandigarh 160012, India
[2] Postgrad Inst Med Educ & Res, Dept Renal Transplant Surg, Chandigarh, India
[3] Postgrad Inst Med Educ & Res, Dept Nephrol, Chandigarh, India
[4] Post Grad Inst Med Educ & Res, Dept Immunopathol, Chandigarh 160012, India
来源：
TRANSPLANTATION PROCEEDINGS | 2023年 / 55卷 / 01期
关键词：
CROSS-MATCH; CYTOTOXICITY;
D O I：
10.1016/j.transproceed.2022.11.007
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Background. Presence of preformed donor specific antibodies (DSAs) detected by complement-dependent cytotoxicity (CDC-XM) is a strong contraindication for transplant. However, it has limitations including its sensitivity and its inability to distinguish between HLA-specific and other non-HLA-specific antibodies. In this study, we standardized CDC-XM by flow cytometry and determined its relevance by comparing its results with other methods of DSA detection, such as routine CDC-XM, antibody binding assay by flow cytometry (FC-XM), and Luminex-based crossmatch assays, such as Luminex crossmatch (LXM) and virtual crossmatch (VXM).Materials and Methods. A total of 79 serum samples were tested for DSAs by the flow cytometric complement-dependent cytotoxicity crossmatch assay (FC-CDC-XM) and then the results of FC-CDC-XM were compared with other detection methods such as CDC-XM, FC-XM, LXM, and VXM.Results. We found that the FC-CDC-XM assay is more sensitive than routine CDC-XM. Out of total 79 sera, 24 sera were detected positive (T cells positive: 1 case and B cells positive: 23) by FC-CDC-XM as compared with 3 sera using CDC-XM; these 3 sera also showed positivity by FC-CDC-XM. After FC-XM assay, 23 samples were positive by FC-XM and out of these 23 samples, 13 were also positive by FC-CDC-XM. On comparing the FC-CDC-XM results with VXM and LXM, 10 sera of 24 FC-CDC-XM positive had HLA class II antibodies detected on aConclusions. The FC-CDC-XM is a more sensitive and specific method for detection of HLA-specific complement-fixing antibodies than CDC-XM and FC-XM. FC-CDC-XM should be used in tissue-typing laboratories after intra- and inter- laboratory validation.
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收藏
页码：134 / 139
页数：6
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