Novel Flow Cytometry-Based Method for Detection of Anti-HLA Complement Activating Donor-Specific Antibodies in Renal Transplant Recipients and Its Comparison With Other Conventional Detection Methods

被引：1
|
作者：
Rani, Lekha [1 ]
Singh, Heera [1 ]
Saikia, Biman [1 ]
Aggarwal, Ritu [1 ]
Kumar, Yashwant [1 ]
Kumar, Mahendra [1 ]
Chhabra, Seema [1 ]
Kumar, Manoj [2 ]
Kumar, Bhuvnesh [2 ]
Kumar, Vinkesh [2 ]
Das, Prabir [1 ]
Sharma, Ashish [2 ]
Ramchandran, Raja [3 ]
Kohli, Harbir Singh [3 ]
Minz, Ranjana Walker [1 ,4 ]
机构：
[1] Postgrad Inst Med Educ & Res, Dept Immunopathol, Chandigarh 160012, India
[2] Postgrad Inst Med Educ & Res, Dept Renal Transplant Surg, Chandigarh, India
[3] Postgrad Inst Med Educ & Res, Dept Nephrol, Chandigarh, India
[4] Post Grad Inst Med Educ & Res, Dept Immunopathol, Chandigarh 160012, India
来源：
TRANSPLANTATION PROCEEDINGS | 2023年 / 55卷 / 01期
关键词：
CROSS-MATCH; CYTOTOXICITY;
D O I：
10.1016/j.transproceed.2022.11.007
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Background. Presence of preformed donor specific antibodies (DSAs) detected by complement-dependent cytotoxicity (CDC-XM) is a strong contraindication for transplant. However, it has limitations including its sensitivity and its inability to distinguish between HLA-specific and other non-HLA-specific antibodies. In this study, we standardized CDC-XM by flow cytometry and determined its relevance by comparing its results with other methods of DSA detection, such as routine CDC-XM, antibody binding assay by flow cytometry (FC-XM), and Luminex-based crossmatch assays, such as Luminex crossmatch (LXM) and virtual crossmatch (VXM).Materials and Methods. A total of 79 serum samples were tested for DSAs by the flow cytometric complement-dependent cytotoxicity crossmatch assay (FC-CDC-XM) and then the results of FC-CDC-XM were compared with other detection methods such as CDC-XM, FC-XM, LXM, and VXM.Results. We found that the FC-CDC-XM assay is more sensitive than routine CDC-XM. Out of total 79 sera, 24 sera were detected positive (T cells positive: 1 case and B cells positive: 23) by FC-CDC-XM as compared with 3 sera using CDC-XM; these 3 sera also showed positivity by FC-CDC-XM. After FC-XM assay, 23 samples were positive by FC-XM and out of these 23 samples, 13 were also positive by FC-CDC-XM. On comparing the FC-CDC-XM results with VXM and LXM, 10 sera of 24 FC-CDC-XM positive had HLA class II antibodies detected on aConclusions. The FC-CDC-XM is a more sensitive and specific method for detection of HLA-specific complement-fixing antibodies than CDC-XM and FC-XM. FC-CDC-XM should be used in tissue-typing laboratories after intra- and inter- laboratory validation.
引用
收藏
页码：134 / 139
页数：6
相关论文
共 37 条
  • [1] Detection of donor-specific anti-HLA antibodies with flow cytometry in eluates and sera from renal transplant recipients with chronic allograft nephropathy
    Martin, L
    Guignier, F
    Mousson, C
    Rageot, D
    Justrabo, E
    Rifle, G
    TRANSPLANTATION, 2003, 76 (02) : 395 - 400
  • [2] CHARACTERISTICS OF DONOR-SPECIFIC ANTI-HLA ANTIBODIES IN RENAL TRANSPLANT RECIPIENTS.
    Ghandorah, Salim
    Yilmaz, Serdar
    Abadi, Abdulnaser
    Rahmanian, Taleb
    Wang, Jinguo
    Khan, Faisal M.
    Berka, Noureddine
    HUMAN IMMUNOLOGY, 2015, 76 : 163 - 163
  • [3] Preexisting anti-HLA donor-specific antibodies in pediatric renal transplant recipients - A new challenge?
    Weber, Lutz T.
    PEDIATRIC TRANSPLANTATION, 2017, 21 (08)
  • [4] IMPACT OF EVEROLIMUS ON DE NOVO DONOR-SPECIFIC ANTI-HLA ANTIBODIES IN RENAL TRANSPLANT RECIPIENTS
    Kitajima, Kumiko
    Nakajima, Ichiro
    Fuchinoue, Shohei
    TRANSPLANT INTERNATIONAL, 2013, 26 : 245 - 245
  • [5] Improved detection of donor preformed specific anti-HLA antibodies in kidney transplants recipients by flow cytometry crossmatching
    Fagundes, I.
    Jobim, M.
    Kulzer, A.
    Chamun, B.
    Gamio, F.
    Ewald, G.
    Schlottfeldt, J.
    Oliveira, F.
    Kruger, M.
    Toresan, R.
    Salim, P. H.
    Lindhal, F.
    Jobim, L. F.
    TISSUE ANTIGENS, 2008, 72 (03): : 259 - 260
  • [6] For Anti-HLA-Specific Donor Antibodies Detection By Flow Cytometry Cytotoxic Crossmatches Comparison of Methods
    Cervelli, C.
    Pisani, F.
    Aureli, A.
    Azzarone, R.
    Scimitarra, M.
    Battistoni, C.
    Di Iulio, B.
    Fracassi, D.
    Scarnecchia, M. A.
    Famulari, A.
    Papola, F.
    TRANSPLANTATION PROCEEDINGS, 2013, 45 (07) : 2761 - 2764
  • [7] Use of cell-free DNA analysis to determine need for renal transplant biopsy after detection of circulating donor-specific anti-HLA antibodies in renal transplant recipients
    Gallay, B. J.
    Suo, L.
    Dvorai, R. Hod
    HUMAN IMMUNOLOGY, 2023, 84 : 89 - 90
  • [8] Clinical implication of non-complement-binding de novo donor-specific anti-HLA antibodies in heart transplant recipients
    Sato, Takuma
    Seguchi, Osamu
    Nakajima, Seiko
    Matsuda, Sachi
    Kanaumi, Yoshiaki
    Wada, Kyoichi
    Yanase, Masanobu
    Ueda, Hatsue
    Fukushima, Norihide
    Nakatani, Takeshi
    TRANSPLANTATION, 2016, 100 (07) : S825 - S825
  • [9] Donor-specific Anti-hla Antibodies At The Time Of Kidney Transplant Biopsy Associate With Late Graft Failure In Pediatric Renal Transplant Recipients
    Alexander, Fichtner
    Caner, SUesal
    Britta, HOecker
    Susi, Rieger
    RUediger, Waldherr
    Gerhard, Opelz
    Burkhard, TOenshoff
    PEDIATRIC NEPHROLOGY, 2014, 29 (09) : 1776 - 1776
  • [10] Donor-Specific Anti-HLA Antibodies at the Time of Kidney Transplant Biopsy Associates With Late Graft Failure in Pediatric Renal Transplant Recipients
    Fichtner, A.
    Hoecker, B.
    Rieger, S.
    Waldherr, R.
    Opelz, G.
    Suesal, C.
    Toenshoff, B.
    AMERICAN JOURNAL OF TRANSPLANTATION, 2014, 14 : 504 - 504
1234