Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells

被引:3
|
作者
Colunga-Saucedo, Mayra [1 ]
Rubio-Hernandez, Edson I. [2 ]
Coronado-Ipina, Miguel A. [3 ]
Rosales-Mendoza, Sergio [4 ,5 ]
Castillo, Claudia G. [2 ]
Comas-Garcia, Mauricio [1 ,3 ,6 ]
机构
[1] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Genom Med, San Luis Potosi 78210, Mexico
[2] Univ Autonoma San Luis Potosi, Fac Med, Lab Celulas Troncales Humanas Coordinac Innovac &, San Luis Potosi 78210, Mexico
[3] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Microscopia Alta Resoluc, San Luis Potosi 78210, Mexico
[4] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Biotecnol, San Luis Potosi 78210, Mexico
[5] Univ Autonoma San Luis Potosi, Fac Ciencias Quim, San Luis Potosi 78210, Mexico
[6] Univ Autonoma San Luis Potosi, Fac Ciencias, San Luis Potosi 78295, Mexico
来源
VIRUSES-BASEL | 2023年 / 15卷 / 01期
关键词
Chikungunya virus; alphavirus DNA vectors; replicon plasmid; helper plasmid; VENEZUELAN EQUINE ENCEPHALITIS; EXPRESSION VECTORS; GENE-THERAPY; IN-VITRO; VACCINE; DELIVERY; GENERATION; IMMUNOGENICITY; ALPHAVIRUSES; REPLICATION;
D O I
10.3390/v15010132
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.
引用
收藏
页数:15
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