Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells

被引：3

作者：

Colunga-Saucedo, Mayra ^{[1
]}

Rubio-Hernandez, Edson I. ^{[2
]}

Coronado-Ipina, Miguel A. ^{[3
]}

Rosales-Mendoza, Sergio ^{[4
,5
]}

Castillo, Claudia G. ^{[2
]}

Comas-Garcia, Mauricio ^{[1
,3
,6
]}

机构：

[1] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Genom Med, San Luis Potosi 78210, Mexico

[2] Univ Autonoma San Luis Potosi, Fac Med, Lab Celulas Troncales Humanas Coordinac Innovac &, San Luis Potosi 78210, Mexico

[3] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Microscopia Alta Resoluc, San Luis Potosi 78210, Mexico

[4] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Biotecnol, San Luis Potosi 78210, Mexico

[5] Univ Autonoma San Luis Potosi, Fac Ciencias Quim, San Luis Potosi 78210, Mexico

[6] Univ Autonoma San Luis Potosi, Fac Ciencias, San Luis Potosi 78295, Mexico

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 01期

关键词：

Chikungunya virus; alphavirus DNA vectors; replicon plasmid; helper plasmid; VENEZUELAN EQUINE ENCEPHALITIS; EXPRESSION VECTORS; GENE-THERAPY; IN-VITRO; VACCINE; DELIVERY; GENERATION; IMMUNOGENICITY; ALPHAVIRUSES; REPLICATION;

D O I：

10.3390/v15010132

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.

引用

页数：15

共 50 条

[1] Construction of adeno-associated virus packaging plasmids and cells that directly select for AAV helper functions
Whiteway, A
Deru, W
Prentice, HG
Anderson, R
JOURNAL OF VIROLOGICAL METHODS, 2003, 114 (01) : 1 - 10
[2] Identification of chikungunya virus interacting proteins in mammalian cells
Pangankar, Mandar S.
Arankalle, Vidya A.
JOURNAL OF BIOSCIENCES, 2014, 39 (03) : 389 - 399
[3] Identification of chikungunya virus interacting proteins in mammalian cells
Mandar S Paingankar
Vidya A Arankalle
Journal of Biosciences, 2014, 39 : 389 - 399
[4] Construction and Identification of the Helper Plasmids for Reverse Genetic System of Rabies Virus Street Strain
Ping-gang MING1
2. Department of Genetic Engineering
3. Laboratory of Epidemiology
Virologica Sinica, 2009, 24 (06) : 559 - 565
[5] Transfection of mammalian cells
Peel, AL
METHODS, 2004, 33 (02) : 93 - 94
[6] RNA interference mediated inhibition of Chikungunya virus replication in mammalian cells
Dash, Paban Kumar
Tiwari, Mugdha
Santhosh, S. R.
Parida, Manmohan
Rao, P. V. Lakshmana
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 376 (04) : 718 - 722
[7] Optical transfection of mammalian cells
Stevenson, David
Agate, Ben
Paterson, Lynn
Lake, Tanya
Comrie, Muriel
Brown, Tom
Riches, Andrew
Bryant, Peter
Sibbett, Wilson
Gunn-Moore, Frank
Dholakia, Kishan
BIOPHOTONICS AND NEW THERAPY FRONTIERS, 2006, 6191
[8] Transfection of mammalian cells by electroporation
不详
NATURE METHODS, 2006, 3 (01) : 67 - 68
[9] Transfection of mammalian cells by electroporation
Nature Methods, 2006, 3 (1) : 67 - 68
[10] Endocytosis of Chikungunya Virus into Mammalian Cells: Role of Clathrin and Early Endosomal Compartments
Bernard, Eric
Solignat, Maxime
Gay, Bernard
Chazal, Nathalie
Higgs, Stephen
Devaux, Christian
Briant, Laurence
PLOS ONE, 2010, 5 (07):

← 1 2 3 4 5 →