MicroED in drug discovery

被引：11

作者：

Danelius, Emma ^{[1
,2
]}

Patel, Khushboo ^{[1
,2
]}

Gonzalez, Brenda ^{[1
,2
]}

Gonen, Tamir ^{[1
,2
,3
]}

机构：

[1] Univ Calif Los Angeles, Dept Biol Chem, 615 Charles E Young Dr South, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Dept Physiol, 615 Charles E Young Dr South, Los Angeles, CA 90095 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2023年 / 79卷

基金：

美国国家卫生研究院;

关键词：

Cr yo-EM MicroED; Small molecule structures; Nanocrystals; Protein-ligand structures; INITIO STRUCTURE DETERMINATION; ELECTRON CRYSTALLOGRAPHY; NANOCRYSTALS; RESOLUTION;

D O I：

10.1016/j.sbi.2023.102549

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cryo-electron microscopy (cryo-EM) method microcrystal electron diffraction (MicroED) was initially described in 2013 and has recently gained attention as an emerging technique for research in drug discovery. As compared to other methods in structural biology, MicroED provides many advantages deriving from the use of nanocrystalline material for the investigations. Here, we review the recent advancements in the field of MicroED and show important examples of small molecule, peptide and protein structures that has contributed to the current development of this method as an important tool for drug discovery.

引用

页数：7

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