Synthesis and Biological Evaluation of Some New Thieno [2, 3-d] Pyrimidine-based Benzodiazepine Derivatives

The goal of our research was to create a new thienopyrimidine-based benzodiazepine derivatives, analyze them biologically, and determine their antioxidant potential. The Aza-Micheal addition reaction was used to synthesize a new series of thieno [2,3-d]pyrimidine-based benzodiazepine derivatives from E-3-(substituted phenyl)-1-(3-((5,6,7,8tetrahydrobenzo [4,5] thieno[2,3-d]pyrimidin-4-yl)amino)phenyl)prop-2-en-1-one(3a-3j) and o-phenylene diamine. DPPH, NO, and H2O2 radical scavenging methods were used to evaluate all of these new compounds for in vitro antioxidant activity. The presence of an electron-donating group on the thienopyrimidine ring improves the activity of compounds 4f and 4i, resulting in significant radical scavenging. Antimicrobial activity was also tested for the synthesized compounds.