AMELIORATED 7,12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED STRESS AND MUTAGENESIS IN RATS

Moringa oleifera and Musa sapientum are ethno-medicinal plants, while 7,12-Dimethylbenz[a] anthracene is a carcinogen. This study evaluated anticancer potentials of MOF6 (extracted from Moringa oleifera leaves) and MSF1 (extracted from Musa sapientum suckers) in 7,12-Dimethylbenz[a]anthracene-induced mutagenesis in rats. Forty-five adult male rats were randomly divided into 9 groups (n = 5). Group 1 was Control. Groups 2 -6 received single intra-peritoneal administration of 15 mg/Kg bodyweight of Dimethylbenz[a]anthracene on Day 1. Groups 3 -6 were post-treated with 15 and 30 mg/Kg bodyweight MOF6 (Days 15 -56), 10 mg/Kg bodyweight MSF1 (Days 15-56) and Doxorubicin/Cisplatin-dose (Days 15 -29) respectively. Groups 7 -9 received only MOF6-doses and MSF1-dose respectively. Doses of 7,12-Dimethylbenz[a]anthracene and extracts were administered orally. Skin histo-pathology (Haematoxylin and Eosin), thiobarbituric acid assay of Malondialdehyde (in Liver homogenates), and ELISA concentrations of sera TNF alpha and Liver homogenates' p53 were evaluated. Data were statistically analyzed (p <= 0.05). Histopathological evaluations showed normal skin histology in Groups 1, 4, 8 and 9. Significant skin histo-alteration was observed in Group 2. Mild skin histo-alterations were observed in Groups 5 and 6. Statistical analyses showed decreased levels of TNF-alpha, Malondialdehyde and p53 in Groups 3 -9 compared with Group 2. Overall, MOF6 and MSF1 ameliorated 7,12-Dimethylbenz[a]anthracene-induced skin histo-pathology, inflammation, hepatic oxidative stress and mutagenesis.