Enantiomeric resolution of ketoprofen using validated thin layer chromatographic method involving frovatriptan as chiral selector

The importance of developing chiral chromatographic methods to monitor and ensure the purity of enantiomers is underscored by the distinct biological properties exhibited by the different forms of ketoprofen. We employed a thin-layer chromatographic approach to separate ketoprofen from commercially available tablet formulations. Our method utilized frovatriptan as the chiral selector, which was incorporated into the stationary phase through a silica gel slurry. The mobile phase consisted of a combination of acetonitrile, methanol, and triethylamine in a ratio of 6:2:2, with a pH of 9. Frovatriptan also served as the chiral selector in the stationary phase, maintaining a pH of 5. The detection and quantitation limits for the ketoprofen enantiomers were determined to be 8.4 mu g spot(-1) and 25.2 mu g spot(-1), respectively. Notably, this study marks the first-ever application of frovatriptan as a chiral selector in thin-layer chromatography to resolve ketoprofen enantiomers.