UTILIZING AUTOENCODERS FOR ANALYSIS AND CLASSIFICATION OF MICROSCOPIC IN SITU HYBRIDIZATION IMAGES

被引：0
|
作者：
Yanev, Aleksandar A. [1 ]
Momcheva, Galina D. [2 ]
Pavlov, Stoyan P. [3 ,4 ]
机构：
[1] High Sch Math Dr Petar Beron, Aleksander Stamboliyski Blvd, Varna 9000, Bulgaria
[2] Bulgarian Acad Sci, Inst Math & Informat, Akad Georgi Bonchev St,Bl 8, Sofia 1113, Bulgaria
[3] Med Univ Varna, Dept Anat & Cell Biol, 55 Prof Marin Drinov St, Varna 9002, Bulgaria
[4] Med Univ Prof Dr Paraskev Stoyanov, Adv Computat Bioimaging Res Inst, Varna, Bulgaria
来源：
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2023年 / 76卷 / 11期
关键词：
artificial neural networks; deep learning autoencoders; image analysis; unsupervised learning; fuzzy clustering;
D O I：
10.7546/CRABS.2023.11.11
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Currently, analysis of microscopic In Situ Hybridization (ISH) images is done manually by experts. Precise evaluation and classification of such micro-scopic images can ease experts' work and reveal further insights about the data. In this work, we propose a deep-learning workflow to detect and classify areas of microscopic images with similar levels of gene expression. Analysis of the data is done by employing a type of ANN - Deep Learning Autoencoders - suitable for unsupervised learning. The model's performance is optimised by balancing the latent layers' length and complexity and fine-tuning hyperparameters. The results are validated by adapting the mean-squared error (MSE) metric and comparison to expert's evaluation. Reconstruction of the whole-scale micro-scopic images is used to summarise and visualise the results.
引用
收藏
页码：1733 / 1742
页数：10
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