Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors

被引：1

作者：

Billeskov, Tine Borum ^{[3
,6
,7
]}

Jensen, Jonas Brorson ^{[1
,2
,3
,4
,5
]}

Jessen, Niels ^{[1
,2
,3
,4
,5
,6
]}

Farup, Jean ^{[1
,2
,3
,4
,5
]}

机构：

[1] Aarhus Univ, Dept Biomed, DK-8000 Aarhus, Denmark

[2] Aarhus Univ, Dept Clin Med, Res Lab Biochem Pathol, DK-8200 Aarhus, Denmark

[3] Aarhus Univ Hosp, Steno Diabet Ctr Aarhus, DK-8200 Aarhus, Denmark

[4] Aarhus Univ Hosp, Dept Nucl Med, DK-8200 Aarhus, Denmark

[5] Aarhus Univ Hosp, PET Ctr, DK-8200 Aarhus, Denmark

[6] Aarhus Univ Hosp, Hormonal & Bone Dis, DK-8200 Aarhus, Denmark

[7] Aarhus Univ Hosp, Dept Clin Pharmacol, DK-8200 Aarhus, Denmark

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cell Biology; Cell Differentiation; Microscopy; Stem Cells; Tissue Engineering;

D O I：

10.1016/j.xpro.2022.102008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies.For complete details on the use and execution of this protocol, please refer to Farup et al. (2021).1

引用

页数：26

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