Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors

被引：1

作者：

Billeskov, Tine Borum ^{[3
,6
,7
]}

Jensen, Jonas Brorson ^{[1
,2
,3
,4
,5
]}

Jessen, Niels ^{[1
,2
,3
,4
,5
,6
]}

Farup, Jean ^{[1
,2
,3
,4
,5
]}

机构：

[1] Aarhus Univ, Dept Biomed, DK-8000 Aarhus, Denmark

[2] Aarhus Univ, Dept Clin Med, Res Lab Biochem Pathol, DK-8200 Aarhus, Denmark

[3] Aarhus Univ Hosp, Steno Diabet Ctr Aarhus, DK-8200 Aarhus, Denmark

[4] Aarhus Univ Hosp, Dept Nucl Med, DK-8200 Aarhus, Denmark

[5] Aarhus Univ Hosp, PET Ctr, DK-8200 Aarhus, Denmark

[6] Aarhus Univ Hosp, Hormonal & Bone Dis, DK-8200 Aarhus, Denmark

[7] Aarhus Univ Hosp, Dept Clin Pharmacol, DK-8200 Aarhus, Denmark

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Cell Biology; Cell Differentiation; Microscopy; Stem Cells; Tissue Engineering;

D O I：

10.1016/j.xpro.2022.102008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of stem cells to activate and differentiate is critical for maintaining the regenerative capacity of skeletal muscle. Here, we detail steps for specific quantification and isolation of primary human fibro-adipogenic progenitors and skeletal muscle stem cells using fluorescence-activated cell sorting. We describe important phenotypic traits such as time to enter the cell cycle and assessment of cell differentiation for the isolated cell populations. The technique has been applied on tissue obtained from surgery and needle biopsies.For complete details on the use and execution of this protocol, please refer to Farup et al. (2021).1

引用

页数：26

共 50 条

[1] The Role of Fibro-Adipogenic Progenitors in Musculoskeletal Disease
Kaur, G.
Davies, M. R.
Liu, X.
Feeley, B. T.
MLTJ-MUSCLES LIGAMENTS AND TENDONS JOURNAL, 2021, 11 (02): : 201 - 214
[2] Metformin inhibits proliferation of fibro-adipogenic progenitors residing in the human heart
Moller, A.
Larsen, A. H.
Lin, L.
Farup, J.
Tolbod, L. P.
Billeskov, T.
Jensen, J. B.
Sundelin, E. I.
Jakobsen, S.
Pedersen, S. B.
DePaoli, F. V.
Luo, Y.
Frokiaer, J.
Wigger, H.
Jessen, N.
DIABETOLOGIA, 2020, 63 (SUPPL 1) : S433 - S433
[3] FLUORESCENCE-ACTIVATED CELL SORTING
HERZENBERG, LA
SWEET, RG
HERZENBERG, LA
SCIENTIFIC AMERICAN, 1976, 234 (03) : 108 - 117
[4] FLUORESCENCE-ACTIVATED CELL SORTING
SIMPSON, E
TRENDS IN BIOCHEMICAL SCIENCES, 1976, 1 (02) : N30 - N31
[5] PURIFICATION OF HUMAN MEGAKARYOCYTES BY FLUORESCENCE-ACTIVATED CELL SORTING
TOMER, A
HARKER, LA
BURSTEIN, SA
BLOOD, 1987, 70 (06) : 1735 - 1742
[6] Fibro-adipogenic progenitors in skeletal muscle homeostasis, regeneration and diseases
Molina, Thomas
Fabre, Paul
Dumont, Nicolas A.
OPEN BIOLOGY, 2021, 11 (12)
[7] Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
Te, Lucas Jaryd Iringan
Doherty, Christina
Correa, Judy
Batt, Jane
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021, (172):
[8] Thrown for a loop: fibro-adipogenic progenitors in skeletal muscle fibrosis
Loomis, Taryn
Smith, Lucas R.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2023, 325 (04): : C895 - C906
[9] SENESCENT FIBRO-ADIPOGENIC PROGENITORS ARE POTENTIAL DRIVERS OF PATHOLOGY IN INCLUSION
Nelke, Christopher
Schroeter, Christina B.
Theissen, Lukas
Preusse, Corinna
Pawlitzki, Marc
Raueber, Saskia
Dobelmann, Vera
Cengiz, Derya
Kleefeld, Felix
Roos, Andreas
Schoser, Benedikt
Brunn, Anna
Neuen-Jacob, Eva
Zschuentzsch, Jana
Meuth, Sven G.
Stenzel, Werner
Ruck, Tobias
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 2024, 42 (02) : 495 - 495
[10] Rotator cuff tear degeneration and the role of fibro-adipogenic progenitors
Agha, Obiajulu
Diaz, Agustin
Davies, Michael
Kim, Hubert T.
Liu, Xuhui
Feeley, Brian T.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 2021, 1490 (01) : 13 - 28

← 1 2 3 4 5 →