Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions

被引：7
|
作者：
Peters, Josh P. [1 ]
Brahms, Arne [2 ]
Janicaud, Vivian [1 ,7 ]
Anikeeva, Maria [1 ]
Peschke, Eva [1 ]
Ellermann, Frowin [1 ]
Ferrari, Arianna [1 ]
Hellmold, Dana [3 ]
Held-Feindt, Janka [3 ]
Kim, Na-mi [4 ]
Meiser, Johannes [5 ]
Aden, Konrad [4 ,6 ]
Herges, Rainer [2 ]
Hoevener, Jan-Bernd [1 ]
Pravdivtsev, Andrey N. [1 ]
机构：
[1] Univ Kiel, Univ Med Ctr Kiel, Mol Imaging North Competence Ctr MOIN CC, Dept Radiol & Neuroradiol,Sect Biomed Imaging, Bot Garten 14, D-24118 Kiel, Germany
[2] Univ Kiel, Otto Diels Inst Organ Chem, Otto Hahn Pl 4, D-24098 Kiel, Germany
[3] Univ Med Ctr Kiel, Dept Neurosurg, Arnold Heller Str 3,House D, D-24105 Kiel, Germany
[4] Univ Kiel, Inst Clin Mol Biol, Rosalind Franklin Str 12, D-24105 Kiel, Germany
[5] Luxembourg Inst Hlth, Dept Canc Res, Canc Metab Grp, 6A Rue Nicolas Ernest Barble, L-1210 Luxembourg, Luxembourg
[6] Univ Med Ctr Kiel, Dept Internal Med 1, Kiel, Germany
[7] Vienna Univ Technol, Karlsplatz 13, A-1040 Vienna, Austria
来源：
SCIENCE ADVANCES | 2023年 / 9卷 / 34期
关键词：
NMR-HYPERPOLARIZATION; N-METHYLTRANSFERASE; RELAXATION; MRI; PHOSPHORIBOSYLTRANSFERASE; EFFICIENT; CELLS; 1-METHYLNICOTINAMIDE; METABOLISM; EXPRESSION;
D O I：
10.1126/sciadv.add3643
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Dissolution dynamic nuclear polarization (dDNP) increases the sensitivity of magnetic resonance imaging by more than 10,000 times, enabling in vivo metabolic imaging to be performed noninvasively in real time. Here, we are developing a group of dDNP polarized tracers based on nicotinamide (NAM). We synthesized 1-N-15-NAM and 1-N-15 nicotinic acid and hyperpolarized them with dDNP, reaching (13.0 +/- 1.9)% N-15 polarization. We found that the lifetime of hyperpolarized 1-N-15-NAM is strongly field- and pH-dependent, with T1 being as long as 41 s at a pH of 12 and 1 T while as short as a few seconds at neutral pH and fields below 1 T. The remarkably short 1-N-15 lifetime at low magnetic fields and neutral pH drove us to establish a unique pH neutralization procedure. Using N-15 dDNP and an inexpensive rodent imaging probe designed in-house, we acquired a N-15 MRI of 1-N-15-NAM (previously hyperpolarized for more than an hour) in less than 1 s.
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页数：15
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