DNA vaccine incorporated poly (lactic-co-glycolic) acid (PLGA) microspheres offer enhanced protection against Aeromonas hydrophila infection

被引:2
|
作者
Thirumalaikumar, Eswaramoorthy [1 ]
Vimal, S. [3 ]
Sathishkumar, Ramamoorthy [1 ]
Ravi, M. [2 ]
Karthick, V. [2 ]
Ramya, S. [2 ]
Thomas, John [4 ]
Kumar, Vinay [5 ]
Kamaraj, Chinnaperumal [6 ]
Citarasu, Thavasimuthu [1 ]
机构
[1] Manonmaniam Sundaranar Univ, Ctr Marine Sci & Technol, Aquat Anim Hlth Lab, Rajakkamangalam 629502, Tamilnadu, India
[2] Sathyabama Inst Sci & Technol, DST Fist Sponsored, Ctr Ocean Res, MoES ESTC Cell, Jeppiaar Nagar,Rajiv Gandhi Salai, Chennai 600119, Tamil Nadu, India
[3] Saveetha Inst Med & Tech Sci, Saveetha Med Coll & Hosp, Dept Biochem, Chennai 602105, Tamilnadu, India
[4] VIT Univ, Ctr Nanobiotechnol, Vellore, Tamil Nadu, India
[5] Saveetha Inst Med & Tech Sci, Saveetha Med Coll & Hosp, Dept Community Med, Chennai 602105, Tamil Nadu, India
[6] SRM Inst Sci & Technol, Interdisciplinary Inst Indian Syst Med, Directorate Res, Kancheepuram, Tamil Nadu, India
关键词
Carassius auratus; DNA vaccine; PLGA microspheres; Outer membrane protein; Aeromonas hydrophila; OUTER-MEMBRANE PROTEIN; NONSPECIFIC IMMUNE PARAMETERS; GOLDFISH CARASSIUS-AURATUS; SALMO-SALAR L; LABEO-ROHITA; DENDRITIC CELLS; ORAL DELIVERY; IN-VITRO; NANOPARTICLES; ANTIGEN;
D O I
10.1016/j.ijbiomac.2023.127182
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Encapsulation of DNA vaccines onto carriers enhances the immunogenicity of an antigen. Specifically, biode-gradable polymers offer sustained release of vaccines which is crucial for any targeted delivery approach. Poly (lactic -co-glycolic) acid (PLGA) microspheres were used to load a DNA vaccine having a targeted gene of outer membrane protein (OMP) of Aeromonas hydrophila to clone and construct a DNA vaccine using a eukaryotic expression vector system (pVAX1-OMP DNA) and delivery in Carassius auratus against A. hydrophila infection. PLGA microspheres were prepared by emulsion technique oil-in-water and characterized by a High-Resolution Scanning Electron Microscope (HR-SEM). The results of PLGA-pVAX1-OMP DNA microspheres shows that average of 100-150 mu m particle size and a loading efficiency (LE) of 68.8 %. Results indicate that C. auratus fed with PLGA-pVAX1-OMP DNA microspheres revealed a significant improvement in innate immune response, which includes, myeloperoxidase activity, respiratory burst and total immunoglobulin level compared with control group fish. The immune-related gene, IL1 beta, IL10, TGF, c-type, and g-type lysozyme also showed signif-icantly higher expression after immunization. Furthermore, dietary supplementation of the PLGA-pVAX1-OMP DNA (G III) group exhibited a significantly higher survival rate (78 %) than the control group of fish. These results help us to understand the of mechanism of DNA vaccine administrated feed through PLGA nanoparticles resistance to infection by regulating systemic and innate immunity in Carassius auratus.
引用
收藏
页数:11
相关论文
共 50 条
  • [41] An Overview of Poly(lactic-co-glycolic) Acid (PLGA)-Based Biomaterials for Bone Tissue Engineering
    Gentile, Piergiorgio
    Chiono, Valeria
    Carmagnola, Irene
    Hatton, Paul V.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2014, 15 (03): : 3640 - 3659
  • [42] Electrospinnability of Poly Lactic-co-glycolic Acid (PLGA): the Role of Solvent Type and Solvent Composition
    Liu, Xiaoli
    Baldursdottir, Stefania G.
    Aho, Johanna
    Qu, Haiyan
    Christensen, Lars Porskj'r
    Rantanen, Jukka
    Yang, Mingshi
    PHARMACEUTICAL RESEARCH, 2017, 34 (04) : 738 - 749
  • [43] In Vitro Degradation of Electrospun Poly(Lactic-Co-Glycolic Acid) (PLGA) for Oral Mucosa Regeneration
    Chor, Ana
    Goncalves, Raquel Pires
    Costa, Andrea Machado
    Farina, Marcos
    Ponche, Arnaud
    Sirelli, Lys
    Schrodj, Gautier
    Gree, Simon
    de Andrade, Leonardo Rodrigues
    Anselme, Karine
    Dias, Marcos Lopes
    POLYMERS, 2020, 12 (08)
  • [44] Electrospinnability of Poly Lactic-co-glycolic Acid (PLGA): the Role of Solvent Type and Solvent Composition
    Xiaoli Liu
    Stefania G. Baldursdottir
    Johanna Aho
    Haiyan Qu
    Lars Porskjær Christensen
    Jukka Rantanen
    Mingshi Yang
    Pharmaceutical Research, 2017, 34 : 738 - 749
  • [45] Release mechanisms from gentamicin loaded poly(lactic-co-glycolic acid) (PLGA) microparticles
    Friess, WF
    Schlapp, M
    JOURNAL OF PHARMACEUTICAL SCIENCES, 2002, 91 (03) : 845 - 855
  • [46] Safety evaluation of poly(lactic-co-glycolic acid)/poly(lactic-acid) microspheres through intravitreal injection in rabbits
    Rong, Xianfang
    Yuan, Weien
    Lu, Yi
    Mo, Xiaofen
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 9 : 3057 - 3068
  • [47] Effect of processing variables on the morphology of electrospun poly(lactic-co-glycolic acid) (PLGA) nanofibers
    Liu Fu-juan
    Guo Rui
    Shen Ming-wu
    Wang Shan-yuan
    Shi Xiangyang
    2008 INTERNATIONAL SYMPOSIUM ON FIBER BASED SCAFFOLDS FOR TISSUE ENGINEERING, PROCEEDINGS, 2008, : 6 - 13
  • [48] Biodegradable poly(lactic-co-glycolic acid) microparticles for injectable delivery of vaccine antigens
    Jiang, WL
    Gupta, RK
    Deshpande, MC
    Schwendeman, SP
    ADVANCED DRUG DELIVERY REVIEWS, 2005, 57 (03) : 391 - 410
  • [49] Liraglutide-loaded poly(lactic-co-glycolic acid) microspheres: Preparation and in vivo evaluation
    Wu, Junzi
    Williams, Gareth R.
    Branford-White, Christopher
    Li, Heyu
    Li, Yan
    Zhu, Li-Min
    EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2016, 92 : 28 - 38
  • [50] PREPARATION OF BIODEGRADABLE POLY(LACTIC-CO-GLYCOLIC) ACID MICROSPHERES AND THEIR INVITRO RELEASE OF TIMOLOL MALEATE
    STURESSON, C
    CARLFORS, J
    EDSMAN, K
    ANDERSSON, M
    INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1993, 89 (03) : 235 - 244