Investigation of virulence factors in Pseudomonas aeruginosa isolates by phenotypic and genotypic methods

Introduction: Pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity, and relationship has been determined between antibiotic resistance and biofilm. The aim of this study was to investigate the virulence factors of Pseudomonas aeruginosa isolates by genotypic and phenotypic methods, as well as whether there is a relationship between other virulence factors and antibiotic resistance.Methods: A total of 80 Pseudomonas aeruginosa strains were sent from various clinics included in the study. Identification and antibiotic resistance profile of isolates were determined by Vitek 2 (Biomerioux, France) automated system. Pseudomonas P agar, Pseudomonas F agar, and motility test medium were used for phenotyping tests. Tox A, Exo S, Plc N, and Las B were evaluated with Real-time PCR (Anatolia, Geneworks, Turkey).Results: The highest rates of antibiotic resistance were observed against imipenem (42.5%) and meropenem (40%). Among the isolates, 81.3% tested positive for Tox A, 30% for Exo S, 32.5% for Plc N, and 42.5% for Las B. Additionally, 70.4% of the isolates tested positive for pyocyanin, 41.3% for pyoverdine, 1.8% for pyorubin, and 8.9% tested negative for pyorubin. No statistically significant difference was found between antibiotic resistance and the presence of virulence factors (p > 0.005).Conclusions: The relationship between antibiotic resistance and virulence factors is controversial. There are studies demonstrating the relationship between virulence factors and antibiotic resistance, as well as studies that indicate the absence of such a relationship. Investigating virulence and antibiotic resistance rates may be important for identifying potential drug targets for subsequent research.