Comparison of Commercial Low Molecular Weight Heparin and Homemade Anti-Xa Calibrators to a Commercial Specific Anti-Xa Calibrator for Plasma Rivaroxaban Quantification by Anti-Xa Oral Anticoagulant Plasma Concentration Chromogenic Assay

被引：1
|
作者：
Divsalar, Bita [1 ,2 ]
Kalantari, Tahereh [1 ,2 ]
Mohebbi, Soheila [1 ,2 ]
Bahmanimehr, Ardeshir [3 ]
Shahsavani, Amin [3 ,4 ]
Borhani-Haghighi, Afshin [5 ]
机构：
[1] Shiraz Univ Med Sci, Sch Paramed Sci, Dept Lab Sci, Div Hematol & Blood Banking, Shiraz, Iran
[2] Shiraz Univ Med Sci, Sch Paramed Sci, Diagnost Lab Sci & Technol Res Ctr, Shiraz, Iran
[3] Shiraz Univ Med Sci, Dastgheib Hosp, PND Res Ctr, Thalassemia & Hemophilia Genet, Shiraz, Iran
[4] Shiraz Univ Med Sci, Hematol Res Ctr, Shiraz, Iran
[5] Shiraz Univ Med Sci, Clin Neurol Res Ctr, Shiraz, Iran
来源：
LABORATORY MEDICINE | 2023年 / 54卷 / 04期
关键词：
Anti-Xa; homemade calibrator made by spiked normal pooled plasma; NPP; laboratory; low molecular weight heparin; rivaroxaban; CLINICAL-PRACTICE; APIXABAN;
D O I：
10.1093/labmed/lmac137
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objective The main concern about measuring the concentration of rivaroxaban by anti-Xa assay in some laboratories is the lack of a commercial specific calibrator in emergencies. Therefore, this study aimed at providing a homemade anti-Xa calibrator and commercial low molecular weight heparin (LMWH) anti-Xa calibrator. Methods The anti-Xa plasma concentration of rivaroxaban was measured in 70 patients using a commercial specific anti-Xa calibrator, a commercial LMWH anti-Xa calibrator, and a homemade anti-Xa calibrator. Results We demonstrated a significant correlation and agreement (P < .001) between LMWH-calibrated anti-Xa and the commercial specific calibrator. A significant correlation (P < .001) was found between homemade calibrated anti-Xa made by normal pooled plasma and that calibrated with a commercial specific drug. The nonspecific homemade and LMWH calibrators had excellent agreement (P < .001) and can be used interchangeably. Conclusion Our data showed that for estimating rivaroxaban concentrations, the LMWH calibrator could be used as an alternative calibrator in the anti-Xa assay.
引用
收藏
页码：400 / 405
页数：6
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