Protocol to induce de novo lipid droplets in Saccharomyces cerevisiae

被引：1
|
作者：
Khatri, Amit [1 ]
Choudhary, Vineet [1 ]
机构：
[1] All India Inst Med Sci AIIMS, Dept Biotechnol, Lipid Metab Lab, New Delhi 110029, India
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金：
英国惠康基金;
关键词：
YEAST; ACYLTRANSFERASE;
D O I：
10.1016/j.xpro.2023.102732
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The mechanisms underlying lipid droplet (LD) biogenesis in the endoplasmic reticulum are not completely understood. Here, we present a protocol for inducing de novo LDs containing either triacylglycerol (TAG) or sterol esters (SE) in Saccharomyces cerevisiae. We describe steps for generating conditional yeast mutants by performing gene knockout and introducing galactose-inducible promoter to produce TAG-or SE-containing LDs. We detail the strategy to generate fluorescent LD marker protein to visualize newly formed droplets by fluorescence microscopy.
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页数：22
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