Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat

被引：10

作者：

Xu, Bai ^{[1
,2
]}

Yin, Man ^{[1
]}

Yang, Yaming ^{[3
]}

Zou, Yunlong ^{[1
,4
]}

Liu, Wenbin ^{[1
]}

Qiao, Lianyong ^{[1
]}

Zhang, Jixiang ^{[1
]}

Wang, Zhan ^{[1
]}

Wu, Yayu ^{[1
]}

Shen, He ^{[5
]}

Sun, Minghan ^{[1
]}

Liu, Weiyuan ^{[1
]}

Xue, Weiwei ^{[1
]}

Fan, Yongheng ^{[1
]}

Zhang, Qi ^{[1
]}

Chen, Bing ^{[1
]}

Wu, Xianming ^{[1
]}

Shi, Ya ^{[1
]}

Lu, Falong ^{[1
]}

Zhao, Yannan ^{[1
]}

Xiao, Zhifeng ^{[1
]}

Dai, Jianwu ^{[1
,5
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China

[2] Jiangnan Univ, Wuxi Sch Med, Wuxi 214122, Peoples R China

[3] Peking Univ Hlth Sci Ctr, Beijing 100191, Peoples R China

[4] Jilin Univ, China Japan Union Hosp, Changchun 130033, Peoples R China

[5] Chinese Acad Sci, Suzhou Inst NanoTech & NanoBion, Div Nanobiomed, Suzhou 215123, Peoples R China

来源：

BIOACTIVE MATERIALS | 2023年 / 23卷

基金：

中国国家自然科学基金;

关键词：

Spinal cord injury; Brain-derived NSPCs; Spinal cord-derived NSPCs; H9 embryonic stem cell-derived NSPCs; Collagen scaffolds; NEURONAL DIFFERENTIATION; STEM/PROGENITOR CELLS; NERVOUS-SYSTEM; GROWTH; OLIGODENDROCYTES; IMMUNOGENICITY; CONNECTIVITY; EXPRESSION; HEDGEHOG; THERAPY;

D O I：

10.1016/j.bioactmat.2022.11.008

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Neural stem progenitor cell (NSPC) transplantation has been regarded as a promising therapeutic method for spinal cord injury (SCI) repair. However, different NSPCs may have different therapeutic effects, and it is therefore important to identify the optimal NSPC type. In our study, we compared the transcriptomes of human fetal brain-derived NSPCs (BNSPCs), spinal cord-derived NSPCs (SCNSPCs) and H9 embryonic stem-cell derived NSPCs (H9-NSPCs) in vitro and subsequently we transplanted each NSPC type on a collagen scaffold into a T8-9 complete SCI rat model in vivo. In vitro data showed that SCNSPCs had more highly expressed genes involved in nerve-related functions than the other two cell types. In vivo, compared with BNSPCs and H9-NSPCs, SCNSPCs exhibited the best therapeutic effects; in fact, SCNSPCs facilitated electrophysiological and hindlimb functional recovery. This study demonstrates that SCNSPCs may be an appropriate candidate cell type for SCI repair, which is of great clinical significance.

引用

页码：300 / 313

页数：14

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