Molecular and cellular imaging of the eye

被引:4
|
作者
Nguyen, Van phuc [1 ]
Zhe, Josh [1 ]
Hu, Justin [1 ]
Ahmed, Umayr [1 ]
Paulus, Yannis m. [1 ,2 ]
机构
[1] Univ Michigan, Dept Ophthalmol & Visual Sci, Ann Arbor, MI 48105 USA
[2] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48105 USA
关键词
OPTICAL COHERENCE TOMOGRAPHY; INDOCYANINE GREEN ANGIOGRAPHY; RETINAL-PIGMENT EPITHELIUM; STEM-CELLS; PHOTOACOUSTIC MICROSCOPY; FLUORESCEIN ANGIOGRAPHY; OCT ANGIOGRAPHY; CHOROIDAL NEOVASCULARIZATION; MACULAR DEGENERATION; DIABETIC-RETINOPATHY;
D O I
10.1364/BOE.502350
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The application of molecular and cellular imaging in ophthalmology has numerous benefits. It can enable the early detection and diagnosis of ocular diseases, facilitating timely intervention and improved patient outcomes. Molecular imaging techniques can help identify disease biomarkers, monitor disease progression, and evaluate treatment responses. Furthermore, these techniques allow researchers to gain insights into the pathogenesis of ocular diseases and develop novel therapeutic strategies. Molecular and cellular imaging can also allow basic research to elucidate the normal physiological processes occurring within the eye, such as cell signaling, tissue remodeling, and immune responses. By providing detailed visualization at the molecular and cellular level, these imaging techniques contribute to a comprehensive understanding of ocular biology. Current clinically available imaging often relies on confocal microscopy, multi -photon microscopy, PET (positron emission tomography) or SPECT (singlephoton emission computed tomography) techniques, optical coherence tomography (OCT), and fluorescence imaging. Preclinical research focuses on the identification of novel molecular targets for various diseases. The aim is to discover specific biomarkers or molecular pathways associated with diseases, allowing for targeted imaging and precise disease characterization. In parallel, efforts are being made to develop sophisticated and multifunctional contrast agents that can selectively bind to these identified molecular targets. These contrast agents can enhance the imaging signal and improve the sensitivity and specificity of molecular imaging by carrying various imaging labels, including radionuclides for PET or SPECT, fluorescent dyes for optical imaging, or nanoparticles for multimodal imaging. Furthermore, advancements in technology and instrumentation are being pursued to enable multimodality molecular imaging. Integrating different imaging modalities, such as PET/MRI (magnetic resonance imaging) or PET/CT (computed tomography), allows for the complementary strengths of each modality to be combined, providing comprehensive molecular and anatomical information in a single examination. Recently, photoacoustic microscopy (PAM) has been explored as a novel imaging technology for visualization of different retinal diseases. PAM is a non-invasive, non -ionizing radiation, and hybrid imaging modality that combines the optical excitation of contrast agents with ultrasound detection. It offers a unique approach to imaging by providing both anatomical and functional information. Its ability to utilize molecularly targeted contrast agents holds great promise for molecular imaging applications in ophthalmology. In this review, we will summarize the application of multimodality molecular imaging for tracking chorioretinal angiogenesis along with the migration of stem cells after subretinal transplantation in vivo. (c) 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
引用
收藏
页码:360 / 386
页数:27
相关论文
共 50 条
  • [21] Molecular imaging and cellular reprogramming in single cells
    Wang, Yingxiao
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2019, 258
  • [22] MR techniques for in vivo molecular and cellular imaging
    Delikatny, EJ
    Poptani, H
    RADIOLOGIC CLINICS OF NORTH AMERICA, 2005, 43 (01) : 205 - +
  • [23] Molecular and cellular approaches to understand and treat some diseases of the eye
    Balasubramanian, D
    CURRENT SCIENCE, 2002, 82 (08): : 948 - 957
  • [24] Molecular and cellular biomarkers in dry eye disease and ocular allergy
    Enriquez-de-Salamanca, Amalia
    Bonini, Stefano
    Calonge, Margarita
    CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY, 2012, 12 (05) : 523 - 533
  • [25] Molecular and cellular approaches to understand and treat some diseases of the eye
    Balasubramanian, D.
    2002, Indian Academy of Sciences (82):
  • [26] Molecular imaging in the eye with photothermal optical coherence tomography
    Skala, Melissa
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2018, 59 (09)
  • [27] Cellular and Molecular Dynamics of Vertebrate Eye Morphogenesis: How Neural Crest Shapes the Embryonic Eye
    Kwan, Kristen M.
    Bryan, Chase D.
    Casey, Macaulie A.
    FASEB JOURNAL, 2019, 33
  • [28] Targeted molecular imaging agents for cellular-scale bimodal imaging
    Manning, HC
    Goebel, T
    Thompson, RC
    Price, RR
    Lee, H
    Bornhop, DJ
    BIOCONJUGATE CHEMISTRY, 2004, 15 (06) : 1488 - 1495
  • [29] Molecular imaging with cellular resolution and molecular specificity using miniaturized microscopes
    Contag, Christopher H.
    CANCER RESEARCH, 2011, 71
  • [30] Combination of cellular imaging and molecular analysis for evaluation of cellular gene therapy of RA
    IH Tarner
    E Neumann
    M Judex
    J Schölmerich
    S Gay
    U Müller-Ladner
    Arthritis Res Ther, 5 (Suppl 1):