A simple point-of-care assay accurately detects anti-spike antibodies after SARS-CoV-2 vaccination

被引：0

作者：

Greene, Sarah E. ^{[1
]}

Huang, Yuefang ^{[2
]}

Kim, Wooseob ^{[3
]}

Liebeskind, Mariel J. ^{[4
]}

Chandrasekaran, Vinay ^{[4
]}

Liu, Zhuoming ^{[5
]}

Deepak, Parakkal ^{[6
]}

Paley, Michael A. ^{[7
]}

Lew, Daphne ^{[8
]}

Yang, Monica ^{[9
]}

Matloubian, Mehrdad ^{[9
]}

Gensler, Lianne S. ^{[9
,10
]}

Nakamura, Mary C. ^{[8
,10
]}

O'Hallaran, Jane A. ^{[2
]}

Presti, Rachel M. ^{[2
]}

Whelan, Sean P. J. ^{[5
]}

Buchser, William J. ^{[4
]}

Kim, Alfred H. J. ^{[3
,7
]}

Weil, Gary J. ^{[2
]}

机构：

[1] Washington Univ, Dept Pediat, Div Infect Dis, Sch Med, 4444 Forest Pk Ave,Rm 4184, St Louis, MO 63110 USA

[2] Washington Univ, Dept Med, Div Infect Dis, St Louis, MO 63130 USA

[3] Washington Univ, Dept Pathol & Immunol, Div Immunobiol, Sch Med, St Louis, MO USA

[4] Washington Univ, Dept Genet, Sch Med, St Louis, MO USA

[5] Washington Univ, Dept Mol Microbiol, Sch Med, St Louis, MO USA

[6] Washington Univ, Dept Med, Div Gastroenterol, Sch Med, St Louis, MO USA

[7] Washington Univ, Dept Med, Div Rheumatol, Sch Med, St Louis, MO USA

[8] Washington Univ, Div Biostat, Sch Med, St Louis, MO USA

[9] Univ Calif San Francisco, Dept Med, Div Rheumatol, San Francisco, CA USA

[10] San Francisco VA Hlth Care Syst, San Francisco, CA USA

来源：

JOURNAL OF CLINICAL VIROLOGY PLUS | 2023年 / 3卷 / 01期

基金：

美国国家卫生研究院;

关键词：

SARS-COV-2; Antibody test; Vaccination; Immunocompromise;

D O I：

10.1016/j.jcvp.2023.100135

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objective: Lateral flow assays (LFA) are sensitive for detecting antibodies to SARS-CoV-2 proteins within weeks after infection. This study tested samples from immunocompetent adults, and those receiving treatments for chronic inflammatory diseases (CID), before and after mRNA SARS-CoV-2 vaccination. Methods: We compared results obtained with the COVIBLOCK Covid-19 LFA to those obtained by anti-spike (S) ELISA. Results: The LFA detected anti-S antibodies in 29 of 29 (100%) of the immunocompetent and 110 of 126 (87.3%) of the CID participants after vaccination. Semiquantitative LFA scores were statistically significantly lower in samples from immunosuppressed participants, and were significantly correlated with anti-S antibody levels measured by ELISA. Conclusions: This simple LFA test is a practical alternative to laboratory-based assays for detecting anti-S antibodies after infection or vaccination. This type of test may be most useful for testing people in outpatient or resource-limited settings.

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页数：5

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