ARHGAP9 knockdown promotes lung adenocarcinoma metastasis by activating Wnt/β-catenin signaling pathway via suppressing DKK2

被引：6
|
作者：
Song, Wenping [1 ,2 ,3 ,4 ]
Wu, Xuan [5 ]
Cheng, Cheng [2 ,6 ]
Li, Ding [1 ,2 ]
Chen, Jinhua [1 ,2 ]
Zhang, Wenzhou [1 ,2 ,7 ,8 ]
机构：
[1] Zhengzhou Univ, Affiliated Canc Hosp, Dept Pharm, Zhengzhou 450008, Peoples R China
[2] Henan Canc Hosp, Zhengzhou 450008, Peoples R China
[3] Henan Canc Hosp, Henan Engn Res Ctr Tumor Precis Med & Comprehens E, Zhengzhou 450008, Peoples R China
[4] Henan Canc Hosp, Henan Prov Key Lab Anticanc Drug Res, Zhengzhou 450008, Peoples R China
[5] Zhengzhou Univ, Peoples Hosp, Dept Resp & Crit Care Med, Zhengzhou 450003, Peoples R China
[6] Zhengzhou Univ, Affiliated Canc Hosp, Dept Hematol, Zhengzhou 450008, Peoples R China
[7] Zhengzhou Univ, Affiliated Canc Hosp, Dept Pharm, 127 Dongming Rd, Zhengzhou 450008, Peoples R China
[8] Henan Canc Hosp, 127 Dongming Rd, Zhengzhou 450008, Peoples R China
来源：
GENOMICS | 2023年 / 115卷 / 05期
基金：
中国国家自然科学基金;
关键词：
ARHGAP9; DKK2; Wnt/beta-catenin signaling pathway; Lung adenocarcinoma; Metastasis; PROTEIN; 9; ARHGAP9; CANCER; GENE; EXPRESSION; NSCLC;
D O I：
10.1016/j.ygeno.2023.110684
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study aims to elucidate the effect of ARHGAP9 on lung adenocarcinoma (LUAD) metastasis, and preliminarily explore its molecular mechanism. As a result, we found that ARHGAP9 was downregulated and correlated with poor prognosis of LUAD. ARHGAP9 knockdown promoted LUAD cell proliferation, migration and invasion, inhibited cell apoptosis and reduced G0G1 cell cycle arrest, in contrast to the results of ARHGAP9 overexpression. Further RNA sequencing analysis demonstrated that ARHGAP9 knockdown in H1299 cells significantly reduced DKK2 (dickkopf related protein 2) expression. Silencing ARHGAP9 in H1299 cells while overexpressing DKK2, DKK2 reversed the promoted effects of ARHGAP9 knockdown on LUAD cell proliferation, migration and invasion. Meanwhile, the activity of Wnt/beta-catenin signaling pathway was also reduced. Taken together, these data indicated that ARHGAP9 knockdown promoted LUAD metastasis by activating Wnt/beta-catenin signaling pathway via suppressing DKK2. This may provide a new strategy for LUAD treatment.
引用
收藏
页数：11
相关论文
共 50 条
  • [31] Long noncoding RNA LINC00673-v4 promotes aggressiveness of lung adenocarcinoma via activating WNT/β-catenin signaling
    Guan, Hongyu
    Zhu, Ting
    Wu, Shanshan
    Liu, Shihua
    Liu, Bangdong
    Wu, Jueheng
    Cai, Junchao
    Zhu, Xun
    Zhang, Xin
    Zeng, Musheng
    Li, Jun
    Song, Erwei
    Li, Mengfeng
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (28) : 14019 - 14028
  • [32] Zinc promotes cell apoptosis via activating the Wnt-3a/β-catenin signaling pathway in osteosarcoma
    Gao, Kai
    Zhang, Yingchun
    Niu, Jianbing
    Nie, Zhikui
    Liu, Qingsheng
    Lv, Chaoliang
    [J]. JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH, 2020, 15 (01)
  • [33] MDIG promotes cisplatin resistance of lung adenocarcinoma by regulating ABC transporter expression via activation of the WNT/β-catenin signaling pathway
    Wang, Qing
    Geng, Feng
    Zhou, Haomin
    Chen, Yecheng
    Du, Juan
    Zhang, Xinyu
    Song, Dandan
    Zhao, Hongwen
    [J]. ONCOLOGY LETTERS, 2019, 18 (04) : 4294 - 4307
  • [34] Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma
    Qian Wu
    Rui Li
    Qing-Xiang Wang
    Meng-Yu Zhang
    Ting-Ting Liu
    Yi-Qing Qu
    [J]. Journal of Translational Medicine, 20
  • [35] Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma
    Wu, Qian
    Li, Rui
    Wang, Qing-Xiang
    Zhang, Meng-Yu
    Liu, Ting-Ting
    Qu, Yi-Qing
    [J]. JOURNAL OF TRANSLATIONAL MEDICINE, 2022, 20 (01)
  • [36] miR-483-3p promotes the osteogenesis of human osteoblasts by targeting Dikkopf 2 (DKK2) and the Wnt signaling pathway
    Zhou, Bin
    Peng, Kun
    Wang, Guoqiang
    Chen, Weihua
    Liu, Ping
    Chen, Fei
    Kang, Yijun
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2020, 46 (04) : 1571 - 1581
  • [37] BMP2 promotes chondrocyte proliferation via the Wnt/β-catenin signaling pathway
    Li, Xihai
    Peng, Jun
    Wu, Mingxia
    Ye, Hongzhi
    Zheng, Chunsong
    Wu, Guangwen
    Xu, Huifeng
    Chen, Xuzheng
    Liu, Xianxiang
    [J]. MOLECULAR MEDICINE REPORTS, 2011, 4 (04) : 621 - 626
  • [38] FBXL20 promotes cell proliferation and metastasis through activating Wnt/β-catenin signaling pathway in esophageal cancer
    He, Feng
    Zhang, Cheng
    Chen, Xiaolong
    Luo, Jun
    Tie, Hongtao
    Li, Qiang
    Wu, Qingchen
    [J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2017, 10 (05): : 7796 - 7805
  • [39] Integrin α6 overexpression promotes lymphangiogenesis and lymphatic metastasis via activating the NF-κB signaling pathway in lung adenocarcinoma
    Ren, Sijia
    Wang, Jing
    Xu, Anyi
    Bao, Jiaqian
    Cho, William C.
    Zhu, Jinrong
    Shen, Jianfei
    [J]. CELLULAR ONCOLOGY, 2022, 45 (01) : 57 - 67
  • [40] Integrin α6 overexpression promotes lymphangiogenesis and lymphatic metastasis via activating the NF-κB signaling pathway in lung adenocarcinoma
    Sijia Ren
    Jing Wang
    Anyi Xu
    Jiaqian Bao
    William C. Cho
    Jinrong Zhu
    Jianfei Shen
    [J]. Cellular Oncology, 2022, 45 : 57 - 67
12345