Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

被引:29
|
作者
Gil-Martinez, Lidia [1 ]
Mut-Salud, Nuria [2 ]
Ruiz-Garcia, Jose Antonio [3 ]
Falcon-Pineiro, Ana [2 ]
Maijo-Ferre, Monica [4 ]
Banos, Alberto [2 ]
De la Torre-Ramirez, Jose Manuel [5 ]
Guillamon, Enrique [6 ]
Verardo, Vito [3 ,7 ]
Gomez-Caravaca, Ana Maria [1 ,7 ]
机构
[1] Univ Granada, Dept Analyt Chem, Avda Fuentenueva, Granada 18071, Spain
[2] Univ Granada, Dept Microbiol, Avda Fuentenueva, Granada 18071, Spain
[3] Univ Granada, Dept Nutr & Food Sci, Campus Cartuja, Granada 18071, Spain
[4] Leitat Technol Ctr, Carrer Innovacio 2, Terrassa 08225, Spain
[5] Univ Jaen, Dept Organ & Inorgan Chem, Jaen 27071, Spain
[6] Univ Granada, Dept Chem Engn, Avda Fuentenueva, Granada 18071, Spain
[7] Univ Granada, Inst Nutr & Food Technol Jose Mataix, Biomed Res Ctr, Avd Conocimiento S-N, Granada 18100, Spain
关键词
Rubus fruticosus; phenolic compounds; terpenic compounds; biological activity; HPLC-MS; TOTAL PHENOLIC CONTENT; FLAVONOL GLYCOSIDES; ETHANOL EXTRACTS; BERRY; WILD; ANTHOCYANINS; POLYPHENOLS; BLUEBERRY; LEAVES; PLANTS;
D O I
10.3390/foods12071505
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds in the extract were ursane-type terpenoids which, along with phenolic compounds, may be responsible for the bioactivity of the extract. In vitro antioxidant capacity was assessed through Folin-Ciocalteu (31.05 +/- 4.9 mg GAE/g d.w.), FRAP (637.8 +/- 3.2 mu mol Fe2+/g d.w.), DPPH (IC50 97.1 +/- 2.4 mu g d.w./mL) and TEAC (576.6 +/- 8.3 mu mol TE/g d.w.) assays. Furthermore, the extract exerted remarkable effects on in vitro cellular antioxidant activity in HUVEC cells at a concentration of 5 mg/mL. Antimicrobial activity of the extract was also tested. Most sensible microorganisms were Gram-positive bacteria, such as E. faecalis, B. cereus and Gram-negative E. coli (MBC of 12.5 mg/mL). IC50 values against colon tumoral cells HT-29 (4.9 +/- 0.2 mg/mL), T-84 (5.9 +/- 0.3 mg/mL) and SW-837 (5.9 +/- 0.2 mg/mL) were also obtained. Furthermore, blackberry extract demonstrated anti-inflammatory activity inhibiting the secretion of pro-inflammatory IL-8 cytokines in two cellular models (HT-29 and T-84) in a concentration-dependent manner. These results support that blackberry fruits are an interesting source of bioactive compounds that may be useful in the prevention and treatment of different diseases, mainly related to oxidative stress.
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页数:18
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