A simple preparation of prestained molecular markers for electrophoresis using inexpensive and readily available proteins

被引：0
|
作者：
Chubatsu, Leda S. [1 ,2 ]
Gerhardt, Edileusa C. M. [1 ]
Souza, Emanuel M. [1 ]
机构：
[1] Univ Fed Parana, Dept Biochem & Mol Biol, Curitiba, PR, Brazil
[2] Univ Fed Parana, Dept Biochem & Mol Biol, CP19046, BR-81531980 Curitiba, PR, Brazil
来源：
ANALYTICAL BIOCHEMISTRY | 2023年 / 676卷
关键词：
Molecular weight markers; Prestained molecular markers; SDS-PAGE markers; OVOTRANSFERRIN; SEPARATION;
D O I：
10.1016/j.ab.2023.115231
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Protein electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate (SDS-PAGE) is one of the most commonly performed procedures in biochemical laboratories. It requires the use of molecular weight (MW) markers as an internal technical control and to determine the migration rate of a particular protein. In this work, we describe a simple method for preparing "homemade" prestained protein markers using readily available cow's milk and chicken egg white proteins without the need of any major protein purification step, and produce prestained MW markers ranging from 19 to 98 kDa.
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页数：4
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