A simple preparation of prestained molecular markers for electrophoresis using inexpensive and readily available proteins

被引：0

作者：

Chubatsu, Leda S. ^{[1
,2
]}

Gerhardt, Edileusa C. M. ^{[1
]}

Souza, Emanuel M. ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Biochem & Mol Biol, Curitiba, PR, Brazil

[2] Univ Fed Parana, Dept Biochem & Mol Biol, CP19046, BR-81531980 Curitiba, PR, Brazil

来源：

ANALYTICAL BIOCHEMISTRY | 2023年 / 676卷

关键词：

Molecular weight markers; Prestained molecular markers; SDS-PAGE markers; OVOTRANSFERRIN; SEPARATION;

D O I：

10.1016/j.ab.2023.115231

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate (SDS-PAGE) is one of the most commonly performed procedures in biochemical laboratories. It requires the use of molecular weight (MW) markers as an internal technical control and to determine the migration rate of a particular protein. In this work, we describe a simple method for preparing "homemade" prestained protein markers using readily available cow's milk and chicken egg white proteins without the need of any major protein purification step, and produce prestained MW markers ranging from 19 to 98 kDa.

引用

页数：4

共 50 条

[1] A SIMPLE INEXPENSIVE AND READILY AVAILABLE AID IN IDENTIFYING GENETIC-MARKERS FOR PSYCHOSES
TURNER, WJ
PSYCHIATRIC JOURNAL OF THE UNIVERSITY OF OTTAWA-REVUE DE PSYCHIATRIE DE L UNIVERSITE D OTTAWA, 1981, 6 (02): : 82 - 85
[2] LAPAROSCOPIC CHOLANGIOGRAPHY - A SIMPLE INEXPENSIVE TECHNIQUE USING READILY AVAILABLE MATERIALS
HARVEY, MH
CAHILL, J
WASTELL, C
BRITISH JOURNAL OF SURGERY, 1992, 79 (11) : 1178 - 1179
[3] LAPAROSCOPIC CHOLANGIOGRAPHY - A SIMPLE INEXPENSIVE TECHNIQUE USING READILY AVAILABLE MATERIALS
KIFF, RS
BRITISH JOURNAL OF SURGERY, 1993, 80 (04) : 538 - 538
[4] Generation of multicolored, prestained molecular weight markers for gel electrophoresis
Compton, MM
Lapp, SA
Pedemonte, R
ELECTROPHORESIS, 2002, 23 (19) : 3262 - 3265
[5] An inexpensive artificial snake hibernaculum built using readily available plumbing supplies
Choquette, Jonathan D.
Savi, Lincoln M.
Fournier, Corentin
METHODSX, 2024, 12
[6] A simple technique for the removal of mottled enamel using readily available materials
R Welbury
British Dental Journal, 1998, 184 (5) : 228 - 228
[7] Simple and Effective Competency Assessment Tracking Using Readily Available Software
Igo, K.
Jones, V.
Figueroa, P.
TRANSFUSION, 2016, 56 : 257A - 257A
[8] Inexpensive and readily available teleendoscopy using a modified pentium PC and a single ISDN2 telephone line
Bell, GD
Rowland, RS
Heatley, DJT
GUT, 1998, 42 : A18 - A18
[9] SEPARATION AND PURIFICATION OF SMALL QUANTITIES OF SPECIFIC RNA SPECIES BY POLYACRYLAMIDE-GEL ELECTROPHORESIS USING PRESTAINED RNAS AS MARKERS
MALHOTRA, LC
MURTHY, MRV
CHAUDHARY, KD
ANALYTICAL BIOCHEMISTRY, 1978, 86 (02) : 363 - 370
[10] p24 antigen capture assay for quantification of human immunodeficiency virus using readily available inexpensive reagents
Wehrly, K
Chesebro, B
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 1997, 12 (04): : 288 - 293

← 1 2 3 4 5 →