Newly Synthesized Arylazo Derivatives Induce Apoptosis and G2/M Cell Cycle Arrest With Molecular Docking Validation in Human Cancer Cell Lines

被引:1
|
作者
Laboud, Yara N. [1 ]
Hassan, Nourhan [2 ,3 ]
Hassaneen, Hamdi M. [1 ]
Hassaneen, Huwaida M. E. [1 ]
Saleh, Fatma M. [1 ]
Teleb, Mohamed A. Mohamed [1 ]
机构
[1] Cairo Univ, Fac Sci, Dept Chem, Giza 12613, Egypt
[2] Munster Univ Hosp, Dept Gynaecol & Obstet, Albert Schweitzer Campus 1, D11, D-48149 Munster, Germany
[3] Cairo Univ, Fac Sci, Dept Biotechnol, Giza 12613, Egypt
关键词
Arylazo derivatives; coupling reaction; cytotoxicity; caspase; 3; cell cycle; apoptosis; REACTIVITY; ANTITUMOR; 1-METHYLISOQUINOLINE; DECOLORIZATION; CYTOTOXICITY; ISOQUINOLINE; SIMULATION;
D O I
10.2174/1871520623666230206105317
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Objective We reported herein the synthesis of novel arylazo derivatives 3a-e incorporating isoquinoline moiety. Methods A coupling reaction of 2-(6,7-dimethoxy-3,4-dihydroisoquinolin-1-yl)acetonitrile 1 with diazotized heterocyclic amines 2 in ethanol in the presence of sodium acetate to give arylazo derivatives 3a-e. Results Cytotoxic effect of five arylazo derivatives on breast carcinoma MCF7 and hepatocellular carcinoma HepG2 was carried out, followed by molecular and functional-based assays, to estimate the anticancer effect of these compounds. The fibroblast growth factor receptor (FGFR) and epithelial growth factor receptor (EGFR) were found to interact and bind with the compounds 3a and 3d through several hydrophobic and hydrogen bonds, which were validated by molecular docking. Conclusion The two promising compounds 3a and 3d demonstrated various anticancer potential activities on tumorigenesis, cytotoxicity, and apoptotic effects, exhibited in the deregulation of the expression of different genes involved in apoptotic and anti-apoptotic mechanisms, cell cycle arrest at G2/M, and induction of apoptosis in both cell lines.
引用
收藏
页码:1192 / 1203
页数:12
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