A new metabolic pathway for sym-homospermidine synthesis in an extreme thermophile, Thermus thermophilus.

被引：0
|
作者：
Oshima, Tairo [1 ]
机构：
[1] Kyowa Kako Co Ltd, Inst Environm Microbiol, 2-15-5 Tadao, Machida, Tokyo 1940035, Japan
来源：
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2023年 / 69卷 / 02期
关键词：
aminopropylagmatinase; deoxyhypusine; synthase; polyamine metabolism; thermophile; Thermus thermophilus; PYRROLIZIDINE ALKALOID BIOSYNTHESIS; DEOXYHYPUSINE SYNTHASE; POLYAMINE BIOSYNTHESIS; EXPRESSION; EVOLUTION; CLONING; IDENTIFICATION; PURIFICATION; BINDING; ENZYME;
D O I：
10.2323/jgam.2023.07.005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In an extreme thermophile, Thermus thermophilus, sym-homospermidine is synthesized by the actions of two enzymes. The first enzyme coded by dhs gene (annotated to be deoxyhypusine synthase gene) catalyzes synthesis of an intermediate, supposed to be 1,9-b i s ( g u a n i d i n o )-5-a z a-n o n a n e (=N1, N11-bis(amidino)-sym-homospermidine), from two molecules of agmatine in the presence of NAD. The second enzyme (aminopropylagmatinase) coded by speB gene catalyzes hydrolysis of the intermediate compound to sym-homospermidine releasing two molecules of urea.
引用
收藏
页码：102 / 108
页数：7
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