Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation

被引：2
|
作者：
Li, Na [1 ,2 ,3 ]
Yan, Yumeng [1 ,2 ,3 ]
Wu, Bowen [1 ,2 ,3 ]
Wang, Jifeng [1 ,2 ,3 ]
Yang, Fuquan [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China
[2] Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100101, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
关键词：
HIGH-RESOLUTION;
D O I：
10.1016/j.xpro.2023.102515
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasma extracellular vesicles (EVs) represent a potential resource for biomarkers of multiple diseases. Here, we present a protocol for obtaining EVs from human plasma using asymmetrical flow field-flow fractionation technology. We describe steps for using tandem mass tags to perform comparative proteomic studies of a large clinical cohort. We then detail targeted quantitative analysis of differential proteins based on a parallel reaction monitoring technique.For complete details on the use and execution of this protocol, please refer to Wu et al. (2020)1 and Li et al. (2023).2
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页数：19
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