Proteomics protocol for obtaining extracellular vesicle from human plasma using asymmetrical flow field-flow fractionation

被引：2

作者：

Li, Na ^{[1
,2
,3
]}

Yan, Yumeng ^{[1
,2
,3
]}

Wu, Bowen ^{[1
,2
,3
]}

Wang, Jifeng ^{[1
,2
,3
]}

Yang, Fuquan ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Key Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Biophys, Lab Prote, Beijing 100101, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

关键词：

HIGH-RESOLUTION;

D O I：

10.1016/j.xpro.2023.102515

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plasma extracellular vesicles (EVs) represent a potential resource for biomarkers of multiple diseases. Here, we present a protocol for obtaining EVs from human plasma using asymmetrical flow field-flow fractionation technology. We describe steps for using tandem mass tags to perform comparative proteomic studies of a large clinical cohort. We then detail targeted quantitative analysis of differential proteins based on a parallel reaction monitoring technique.For complete details on the use and execution of this protocol, please refer to Wu et al. (2020)1 and Li et al. (2023).2

引用

页数：19

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