CHAF1A promotes the proliferation and growth of epithelial ovarian cancer cells by affecting the phosphorylation of JAK2/STAT3 signaling pathway

被引：3
|
作者：
Xia, Dandan [1 ]
Xu, Xun [2 ]
Wei, Jing [1 ]
Wang, Wenli [1 ]
Xiong, Jiali [1 ]
Tan, Qingqing [1 ]
Xue, Pingping [3 ,5 ]
Wang, Huiyan [1 ,4 ]
机构：
[1] Nanjing Med Univ, Changzhou Maternal & Child Hlth Care Hosp, Changzhou Med Ctr, Dept Obstet & Gynecol, Changzhou 213000, Peoples R China
[2] Nanjing Med Univ, Changzhou Maternal & Child Hlth Care Hosp, Changzhou Med Ctr, Dept Orthoped, Changzhou 213000, Peoples R China
[3] Nanjing Med Univ, Changzhou Maternal & Child Hlth Care Hosp, Dept Reprod Med Ctr, Changzhou Med Ctr, Changzhou 213000, Peoples R China
[4] 16 DingXiang Rd, Changzhou 213000, Peoples R China
[5] Changzhou Maternal & Child Hlth Care Hosp, Dept Reprod Med Ctr, Changzhou 213000, Peoples R China
来源：
BIOCHEMISTRY AND BIOPHYSICS REPORTS | 2023年 / 35卷
关键词：
CHAF1A; Epithelial ovarian cancer; JAK2; STAT3; Proliferation; Apoptosis; OVER-EXPRESSION; TRANSCRIPTION; APOPTOSIS;
D O I：
10.1016/j.bbrep.2023.101522
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The molecular mechanism of chromatin assembly factor 1 unit A (CHAF1A) promoting the proliferation and growth of epithelial ovarian cancer (EOC) cells hasn't been reported at present. In this study, recombinant CHAF1A siRNA/overexpression plasmid (si-RNA1/pcDNA3.1-CHAF1A) was designed and constructed, and stable cell lines with knockdown or overexpression of CHAF1A were constructed. The changes of JAK2/STAT3 pathway were detected by Western blot. JAK2/STAT3 pathway was inhibited by Peficitinib, and then cell proliferation and growth ability were detected. Bioinformatics analysis suggested that CHAF1A was up-regulated in epithelial ovarian cancer. JAK2/STAT3 pathway phosphorylation was inhibited in si-RNA1 group, while it was increased in pcDNA3.1-CHAF1A group. After inhibiting JAK2/STAT3 pathway, the promoting effect of CHAF1A on epithelial ovarian cancer cell proliferation disappeared, meanwhile the inhibitory effect of CHAF1A on apoptosis enhanced. In conclusion, CHAF1A promotes the proliferation and growth of epithelial ovarian cancer cells by affecting the phosphorylation of JAK2/STAT3 signaling pathway.
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页数：6
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