MODIFICATION OF TRYPTOPHAN RESIDUES OF IgGRF AND ITS EFFECT ON THE ADSORPTION CAPACITY

被引：0
|
作者：
WANG Lianyong LU Jing YU Yaoting** Bioactive Materials Research Key Laboratory
机构：
来源：
Chinese Journal of Reactive Polymers | 2002年 / 01期
关键词：
immunoadsorbent; IgGRF; immobilized DNA; modification;
D O I：
暂无
中图分类号：
O647.3 [吸附];
学科分类号：
摘要：
Tryptophan residues of IgGRF from rheumatoid arthritis patient were modified with N-Bromosuccinimide (NBS) and the product of modification was characterized by UV-spectrum、Fluorescence emission spectrum and CD-spectrum. The corresponding adsorption capacity of immobilized ssDNA immunoadsorbent for IgGRF was enhanced from 46% to 86%, This result indicates the tryptophan residue is essential for the interaction between ssDNA and IgGRF .and ionic-bonding plays an important role in adsorption.
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页码：49 / 54
页数：6
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