The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection

被引：0

作者：

SHENG Ya Jie ^{[1
]}

EREMIN Sergei ^{[2
]}

MI Tie Jun ^{[1
]}

ZHANG Su Xia ^{[1
]}

SHEN Jian Zhong ^{[1
]}

WANG Zhan Hui ^{[1
]}

机构：

[1] Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety

[2] Department of Chemistry, M.V. Lomonosov Moscow State University

来源：

Biomedical and Environmental Sciences | 2014年 / 27卷 / 02期

关键词：

FPIA; AFB; The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection; AFM; EDF;

D O I：

暂无

中图分类号：

R114 [卫生毒理];

学科分类号：

100405 ;

摘要：

A fluorescence polarization immunoassay(FPIA)was developed for the analysis ofaflatoxins(AFs)using an anti-aflatoxin B1(AFB1)monoclonal antibody and a novel fluorescein-labeled AFB1tracer.The FPIA showed an IC50 value of 23.33ng/mL with a limit of detection of 13.12 ng/mL for AFB1.The cross-reactivities of AFB1,AFB2,AFG1,AFG2,AFM1,and AFM2 with the antibody were100%,65.7%,143%,23.5%,111.4%,and 2%,respectively.The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs,albeit not AFG2 and AFM2.The total time required for analyzing 96 samples in one microplate was less than 5 min.This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.

引用

页码：126 / 129

页数：4

共 50 条

[21] Development of fluorescence polarization immunoassay for the detection of organophosphorus pesticides parathion and azinphos-methyl
Tang, Jianshe
Zhang, Min
Cheng, Guohua
Lu, Yitong
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, 2008, 29 (04): : 356 - 369
[22] Development of a fluorescence polarization immunoassay for the routine detection of N-desmethylzopiclone in urine samples
Mannaert, E
Daenens, P
ANALYST, 1996, 121 (06) : 857 - 861
[23] DEVELOPMENT OF A TDX/ADX FLUORESCENCE POLARIZATION IMMUNOASSAY FOR THE DETECTION OF METHADONE IN HUMAN-URINE
THACKER, S
DUBLER, R
WANG, N
WALLING, J
CHIAPPETTA, E
GATES, T
CRISAFULLI, T
CLINICAL CHEMISTRY, 1989, 35 (06) : 1176 - 1176
[24] FLUORESCENCE POLARIZATION IMMUNOASSAY
JOLLEY, ME
CLINICAL CHEMISTRY, 1981, 27 (06) : 972 - 973
[25] Development of a fluorescence polarization immunoassay for polycyclic aromatic hydrocarbons
Goryacheva, Irina Yu
Eremin, Sergei A.
Shutaleva, Ekaterina A.
Suchanek, Miloslav
Niessner, Reinhard
Knopp, Dietmar
ANALYTICAL LETTERS, 2007, 40 (07) : 1445 - 1460
[26] Development and optimization of a fluorescence polarization immunoassay for orbifloxacin in milk
Mi, Tiejun
Liang, Xiao
Ding, Long
Zhang, Suxia
Eremin, Sergei A.
Beier, Ross C.
Shen, Jianzhong
Wang, Zhanhui
ANALYTICAL METHODS, 2014, 6 (11) : 3849 - 3857
[27] Development of single reagent for fluorescence polarization immunoassay of atrazine
Choi, MJ
Lee, JR
Eremin, SA
FOOD AND AGRICULTURAL IMMUNOLOGY, 2002, 14 (02) : 107 - 120
[28] Development of a fluorescence polarization immunoassay for lorazepam quantification.
Agbuya, PG
Li, L
Miles, MV
Zaritsky, AL
Morris, AD
JOURNAL OF INVESTIGATIVE MEDICINE, 1996, 44 (01) : A60 - A60
[29] Development of a Fluorescence Polarization Immunoassay for the Quantification of Tylosin in Honey
Eremin, S. A.
Mukhametova, L. I.
Arutyunyan, D. A.
Tereshchenkov, A. G.
Sumbatyan, N. V.
Priima, A. D.
Nesterenko, I. S.
Berlina, A. N.
Sotnikov, D. V.
JOURNAL OF ANALYTICAL CHEMISTRY, 2024, 79 (02) : 154 - 161
[30] Potential of fluorescence polarization immunoassay for the detection of Aspergillus fumigatus galactomannan
Krylov, V. B.
Petruk, M. I.
Karimova, M. P.
Mukhametova, L. I.
Matveev, A. L.
Tikunova, N. V.
Eremin, S. A.
Nifantiev, N. E.
RUSSIAN CHEMICAL BULLETIN, 2019, 68 (12) : 2365 - 2369

← 1 2 3 4 5 →