The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection

被引：0
|
作者：
SHENG Ya Jie [1 ]
EREMIN Sergei [2 ]
MI Tie Jun [1 ]
ZHANG Su Xia [1 ]
SHEN Jian Zhong [1 ]
WANG Zhan Hui [1 ]
机构：
[1] Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety
[2] Department of Chemistry, M.V. Lomonosov Moscow State University
来源：
Biomedical and Environmental Sciences | 2014年 / 27卷 / 02期
关键词：
FPIA; AFB; The Development of A Fluorescence Polarization Immunoassay for Aflatoxin Detection; AFM; EDF;
D O I：
暂无
中图分类号：
R114 [卫生毒理];
学科分类号：
100405 ;
摘要：
A fluorescence polarization immunoassay(FPIA)was developed for the analysis ofaflatoxins(AFs)using an anti-aflatoxin B1(AFB1)monoclonal antibody and a novel fluorescein-labeled AFB1tracer.The FPIA showed an IC50 value of 23.33ng/mL with a limit of detection of 13.12 ng/mL for AFB1.The cross-reactivities of AFB1,AFB2,AFG1,AFG2,AFM1,and AFM2 with the antibody were100%,65.7%,143%,23.5%,111.4%,and 2%,respectively.The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs,albeit not AFG2 and AFM2.The total time required for analyzing 96 samples in one microplate was less than 5 min.This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.
引用
收藏
页码：126 / 129
页数：4
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