Application of Box-Behnken Design for Optimization of Different Pretreatment Conditions for Cellulase Production

Effects of dilute acid and acid steam pretreatments were inspected for cellulose production of Eucalyptus leaves through Box-Behenken design,a three variable factors for response surface methodology by Bacillus subtilus K;8.Maximum cellulose production performed in 250 mL erlenmeyer flask with submerged fermentation attained at 50℃,p H 5,140 r·min;for 24 h.Results showed the efficient cellulose production from acid steam pretreatment（being autoclaved at 15 Psi for 15 min）than acid pretreatment.The optimum condition for maximum carboxymethyl cellulas（CMCase）was 1.811 IU·mL;·min;（0.8%acid conc.,10 g biomass loading,6 h reaction time）and filter paper activity（FPase）was 2.255 IU·mL;·min;（1%acid conc.,10 g biomass loading,8 h reaction time）.Whereas,the acid steam maximum CMCase activity recorded was 2.585 IU·mL;·min;（0.8%acid conc.,15 g substrate loading and 8 h reaction time）and the highest FPase activity was 2.055 IU·mL;·min;（0.8%conc.,10 g biomass,6 h reaction time then autoclaved）.Results revealed that acid pretreated Eucalyptus leaves were better lignocellulosic biomass for cellulose production by submerged fermentation.