A novel method of DNA shuffling without PCR process

被引：0
|
作者：
WANG Qiang
机构：
来源：
ChineseScienceBulletin | 2004年 / 07期
关键词：
DNA shuffling; restriction enzyme; tissue plasminogen activator;
D O I：
暂无
中图分类号：
Q52 [核酸];
学科分类号：
071010 ; 081704 ;
摘要：
Most DNA shuffling methods currently used require PCR process. A novel method of DNA shuffling without PCR process is described, taking advantage of the feature of some restriction enzymes whose recognition sites differ from their cleavage sites, thus giving rise to different cohesive ends.These cohesive ends can be rejoined at their native sites from different parental sequences, generating new sequences with various combinations of mutations.
引用
收藏
页码：689 / 691
页数：3
相关论文
共 50 条
  • [31] Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments
    Daxing Wen
    Chunqing Zhang
    Plant Methods, 8
  • [32] DEVELOPMENT OF A NOVEL METHOD FOR OPISTHORCHIS VIVERRINI DNA DETECTION IN URINE BY PCR ASSAY
    Ancharski, Andrew
    Sithithaworn, Paiboon
    Worasith, Chanika
    Kiatsopit, Nadda
    Carlton, Elizabeth
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2017, 95 (05): : 178 - 179
  • [33] DNA shuffling method for generating highly recombined genes and evolved enzymes
    Wayne M. Coco
    William E. Levinson
    Michael J. Crist
    Harm J. Hektor
    Aldis Darzins
    Philip T. Pienkos
    Charles H. Squires
    Daniel J. Monticello
    Nature Biotechnology, 2001, 19 : 354 - 359
  • [34] An effective family shuffling method using single-stranded DNA
    Kikuchi, M
    Ohnishi, K
    Harayama, S
    GENE, 2000, 243 (1-2) : 133 - 137
  • [35] DNA shuffling method for generating highly recombined genes and evolved enzymes
    Coco, WM
    Levinson, WE
    Crist, MJ
    Hektor, HJ
    Darzins, A
    Pienkos, PT
    Squires, CH
    Monticello, DJ
    NATURE BIOTECHNOLOGY, 2001, 19 (04) : 354 - 359
  • [36] Direct PCR from whole blood by using novel buffer without DNA isolation.
    Nakayama, T
    Tonoike, H
    Kojima, K
    Kato, S
    Ueda, K
    CLINICAL CHEMISTRY, 2000, 46 (06) : A18 - A18
  • [37] Computer assisted parental sequences analysis as a previous step to DNA shuffling process
    Montera, Luciana
    do Carmo Nicoletti, Maria
    Henrique-Silva, Flavio
    2006 IEEE CONGRESS ON EVOLUTIONARY COMPUTATION, VOLS 1-6, 2006, : 2300 - 2307
  • [38] Anticipatory evolution and DNA shuffling
    Jamie M Bacher
    Brian D Reiss
    Andrew D Ellington
    Genome Biology, 3 (8)
  • [39] Protein evolution by "codon shuffling": A novel method for generating highly variant mutant libraries by assembly of hexamer DNA duplexes
    Chopra, S
    Ranganathan, A
    CHEMISTRY & BIOLOGY, 2003, 10 (10): : 917 - 926
  • [40] A novel method for the production of metal powders without conventional atomization process
    Canakci, A.
    Varol, T.
    JOURNAL OF CLEANER PRODUCTION, 2015, 99 : 312 - 319
12345